Jun 3, 1975

Messenger RNA complexity in Drosophila melanogaster

L W Beatriz, B J McCarthy


Complementary DNA was synthesized as a copy of polyadenylated RNA from the cytoplasm of Drosophila cultured cells. The kinetics of hybridization of cDNA with the RNA used as template revealed a complex distribution of frequencies in the population of polyadenylated RNA. Computer simulation suggested three frequency classes containing about 4, 190, and 6700 different RNA molecules of mean molecular weight 4 x 10-5. About 15% of this complementary DNA reacted with repetitive sequences of Drosophila DNA. The most frequent polyadenylated RNA is preferentially enriched in its content of repetitive sequences. Comparative experiments using cDNA synthesized as a complement of larval polyadenylated RNA demonstrated some stage specific changes in the population of polyadenylated RNA.

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Mentioned in this Paper

Centrifugation, Density Gradient
Repetitive Region
Nucleic Acid Hybridization Procedure
Transcription, Genetic
RNA, Polyadenylated
Alkaline DNase
Genomic Hybridization
DNA, Complementary

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