Messenger RNA deadenylylation precedes decapping in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America
P CouttetT Grange

Abstract

In yeast, the major mRNA degradation pathway is initiated by poly(A) tail shortening that triggers mRNA decapping. The mRNA is then degraded by 5'-to-3' exonucleolysis. In mammalian cells, even though poly(A) tail shortening also precedes mRNA degradation, the degradation pathway has not been elucidated. We have used a reverse transcription-PCR approach that relies on mRNA circularization to measure the poly(A) tail length of four mammalian mRNAs. This approach allows for the simultaneous analysis of the 5' and 3' ends of the same mRNA molecule. For all four mRNAs analyzed, this strategy permitted us to demonstrate the existence of small amounts of decapped mRNA species which have a shorter poly(A) tail than their capped counterparts. Kinetic analysis of one of these mRNAs indicates that the decapped species with a short poly(A) tail are mRNA degradation products. Therefore, our results indicate that decapping is preceded by a shortening of the poly(A) tail in mammalian cells, as it is in yeast, suggesting that this mRNA degradation pathway is conserved throughout eukaryotic evolution.

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J N VournakisF C Kafatos
Nov 1, 1992·Genes & Development·J E LowellA B Sachs
Jul 1, 1992·Genes & Development·F J SallésS Strickland
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Mar 1, 1983·The Journal of Cell Biology·B W Baer, R D Kornberg
Dec 1, 1995·Genes & Development·S Z Tarun, A B Sachs
Jun 1, 1995·PCR Methods and Applications·F J Sallés, S Strickland
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E BertrandT Grange
Aug 13, 1993·Cell·A B Sachs
Apr 21, 1995·Cell·C A Beelman, R Parker
Apr 1, 1995·Molecular and Cellular Biology·D MuhlradR Parker
Aug 18, 1994·Nature·D Muhlrad, R Parker
Apr 1, 1994·Molecular and Cellular Biology·C E DabrowskiP A Schaffer
Apr 11, 1993·Nucleic Acids Research·M Fromont-RacineT Grange
Jul 1, 1996·Trends in Biochemical Sciences·E Wahle, W Keller

❮ Previous
Next ❯

Citations

Jul 13, 2002·Arthritis and Rheumatism·Seth A BrooksWilliam F C Rigby
Mar 17, 2004·Gene·Yukako ChibaPamela J Green
Nov 30, 2002·Biochimie·Hélène TourrièreJamal Tazi
Mar 20, 2001·Gene·J Guhaniyogi, G Brewer
Oct 25, 2001·Gene·C I GonzálezS W Peltz
Dec 5, 1998·The International Journal of Biochemistry & Cell Biology·I E SchefflerS Prieto
Aug 25, 2009·Nature·Wenqian HuJeff Coller
Apr 16, 2010·Nature·Tae-Kyung KimMichael E Greenberg
May 12, 2009·Nature Structural & Molecular Biology·Olivia S Rissland, Chris J Norbury
Oct 3, 1999·European Journal of Biochemistry·C M Uhlar, A S Whitehead
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Weiping ZhangLeslie E Sieburth
Apr 2, 2011·The Journal of Biological Chemistry·Carsten BoeslerChristian Hammann
Apr 18, 1998·The EMBO Journal·T E LaGrandeur, R Parker
Mar 4, 2000·The EMBO Journal·E DehlinE Wahle
Aug 29, 2002·The EMBO Journal·Hudan LiuMegerditch Kiledjian
Jun 19, 2001·Nucleic Acids Research·M C DaugeronB Séraphin
Jul 26, 2002·Nucleic Acids Research·Séverine ChambeyronIsabelle Busseau
Nov 7, 2003·Nucleic Acids Research·Eugenia BasyukEdouard Bertrand
Jan 27, 2004·Nucleic Acids Research·P Couttet, T Grange
Jan 5, 2006·Nucleic Acids Research·Carine BarreauH Beverley Osborne
Jul 15, 2009·Nucleic Acids Research·Angela SchwedeChristine Clayton
Sep 1, 2005·Genes & Development·Manabu YoshikawaR Scott Poethig
Jul 25, 2013·The Plant Cell·Kestrel Rogers, Xuemei Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.