Messenger RNA stability of parathyroid hormone-related protein regulated by transforming growth factor-beta1

Molecular and Cellular Endocrinology
Rani S SellersThomas J Rosol

Abstract

Humoral hypercalcemia of malignancy (HHM), a paraneoplastic syndrome associated with epithelial cancers, including squamous cell carcinoma (SCC), is due to expression and secretion of parathyroid hormone-related protein (PTHrP). Transforming growth factor-beta1 (TGFbeta1), expressed by many tumors, has been demonstrated in vitro to increase the half-life of PTHrP mRNA. In this study, oral squamous carcinoma cells (SCC2/88) had a two-fold increase in PTHrP mRNA stability (from 45 to 90 min) in response to treatment with TGFbeta1. In order to examine the mechanism of TGFbeta1-mediated PTHrP mRNA stability, a cell-free assay of mRNA degradation was utilized in which the degradation of in vitro-transcribed mRNA incubated with cytoplasmic protein extracts from SCC2/88 treated with vehicle or TGFbeta1 was measured. In this assay, full-length PTHrP mRNA was not significantly stabilized in TGFbeta1-treated samples when compared to vehicle treated samples. However, there was a striking (>5-fold) increase in PTHrP mRNA half-life in TGFbeta1-treated samples when PTHrP mRNA lacked the 3'-untranslated region (3'-UTR). In contrast, the degradation of 3'-UTR-truncated PTHrP mRNA using the cell-free assay was not altered in vehicle-treated sam...Continue Reading

References

Sep 15, 1989·Annals of Internal Medicine·T MotokuraE Ogata
Dec 1, 1989·The Journal of Endocrinology·H M DochertyD A Heath
Jul 1, 1989·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·S K AbbasA D Care
Apr 21, 1995·Cell·C A Beelman, R Parker
Apr 1, 1995·Molecular and Cellular Biology·A M ZubiagaM E Greenberg
Aug 1, 1993·Molecular and Cellular Biology·C L WellingtonJ G Belasco
Sep 1, 1993·Experimental Cell Research·J R WerkmeisterT J Rosol
Nov 1, 1995·Trends in Biochemical Sciences·C Y Chen, A B Shyu
Jan 1, 1996·Physiological Reviews·W M PhilbrickA F Stewart
Nov 5, 1997·The Journal of Biological Chemistry·C T DeMariaG Brewer
Jan 28, 1998·Journal of Virology·M SokolowskiS Schwartz
Apr 29, 1998·Annual Review of Physiology·J J Wysolmerski, A F Stewart
Dec 18, 1998·The Journal of Biological Chemistry·G Brewer
Feb 20, 1999·The Journal of Biological Chemistry·A S JarrousseH P Schmid
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·L P Ford, J Wilusz
Mar 24, 1999·Trends in Genetics : TIG·A J CarpousisL C Raynal
Apr 8, 2000·Current Opinion in Genetics & Development·P Mitchell, D Tollervey

❮ Previous
Next ❯

Citations

Sep 21, 2007·Breast Cancer Research and Treatment·Jennifer L GilmoreJohn Foley
May 23, 2006·Clinical & Experimental Metastasis·S H Tannehill-GreggT J Rosol
Oct 13, 2010·Human Reproduction·Ozgur Oktem, Bulent Urman
Jul 19, 2005·Critical Reviews in Eukaryotic Gene Expression·Virgile RichardJohn Foley
Aug 5, 2004·Journal of Molecular Endocrinology·R S SellersT J Rosol
Jun 7, 2006·The Journal of Pathology·L HameetmanP C W Hogendoorn
Jan 6, 2006·Growth Factors·Alexander DibrovFrancis M Amara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Isuru GajanayakeOliver A Garden
Molecular and Cellular Endocrinology
Veronica A Tovar Sepulveda, Miriam Falzon
© 2022 Meta ULC. All rights reserved