Met-Arg-Trp derived from Rubisco lowers blood pressure via prostaglandin D(2)-dependent vasorelaxation in spontaneously hypertensive rats

Peptides
Hui ZhaoMasaaki Yoshikawa

Abstract

Met-Arg-Trp (MRW) has been isolated as an inhibitor for angiotensin I-converting enzyme (ACE) from a pepsin-pancreatin digest of spinach ribulose bisphosphate carboxylase/oxygenase (Rubisco) (IC(50)=0.6 microM). It has been reported that hypotensive activity of ACE-inhibitory peptides derived from food proteins are weakened in spontaneously hypertensive rats older than 25 weeks (old SHR). However, MRW reduced blood pressure after oral administration at a dose of 5 mg/kg in old SHR as well as in younger SHR. MRW exhibited vasorelaxing activity above 1 microM in isolated mesenteric artery from adult and old SHR. The vasorelaxing activity of MRW was blocked by indomethacin and BW A868C, a cyclooxygenase inhibitor and an antagonist for DP(1) receptor, respectively. However, N(G)-nitro-L-arginine methyl ester, an inhibitor for nitric oxide synthase, had no effect on the relaxation. The hypotensive activity of MRW was also blocked by indomethacin and BW A868C, respectively, in adult and old SHR. Taken together, the vasorelaxing and hypotensive activities of MRW may be mediated by prostaglandin D(2) and the DP(1) receptor. These findings suggest that the hypotensive activity of MRW is mainly caused by vasorelaxation rather than by ACE...Continue Reading

References

Oct 1, 1986·Japanese Circulation Journal·S MochizukiM Nagano
May 1, 1994·Bioscience, Biotechnology, and Biochemistry·Y SaitoS Imayasu
Jun 8, 1999·The Journal of Clinical Investigation·S L TilleyB H Koller
Aug 9, 2003·Journal of Agricultural and Food Chemistry·Yanjun YangMasaaki Yoshikawa
Sep 2, 2003·Peptides·Ewa D MarczakMasaaki Yoshikawa
Apr 15, 2004·Journal of Agricultural and Food Chemistry·Yanjun YangMasaaki Yoshikawa
Jun 7, 2006·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Yoshitomo MoriTadaaki Iwasaki

❮ Previous
Next ❯

Citations

Feb 26, 2013·Journal of Agricultural and Food Chemistry·Masahiro KoyamaKozo Nakamura
Dec 31, 2014·International Journal of Molecular Sciences·Kaustav Majumder, Jianping Wu
Jul 6, 2010·Peptides·Ricardo Fernández-MusolesJuan B Salom
Sep 5, 2012·Molecular Nutrition & Food Research·Tomomi KagebayashiKousaku Ohinata
Oct 10, 2013·Molecular Nutrition & Food Research·Noriyasu KontaniKousaku Ohinata
Dec 3, 2014·Food Chemistry·Akio YamadaFumiaki Abe
Jul 10, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kentaro KanekoKousaku Ohinata
Oct 4, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yukiha MoriKousaku Ohinata
Jul 30, 2019·Journal of Food Biochemistry·Dominic AgyeiMichael K Danquah
Feb 3, 2021·Comprehensive Reviews in Food Science and Food Safety·Lu XuePangzhen Zhang
Mar 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Anna IwaniakMałgorzata Darewicz
May 24, 2021·Peptides·Naohisa Shobako

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antihypertensive Agents: Mechanisms of Action

Antihypertensive drugs are used to treat hypertension (high blood pressure) which aims to prevent the complications of high blood pressure, such as stroke and myocardial infarction. Discover the latest research on antihypertensive drugs and their mechanism of action here.