Met-enkephalin alteration in the rat during chronic injection of morphine and/or midazolam

Brain Research
G A Tejwani, A K Rattan

Abstract

We have recently reported that the short-acting anesthetic and analgesic drug midazolam can produce analgesia and decrease morphine tolerance and dependence in the rat by interacting with the opioid system. This study was designed to investigate the effect of midazolam, morphine, and both together on met-enkephalin levels in the rat. Male Sprague-Dawley rats were divided into four groups: (1) saline-saline; (2) saline-morphine; (3) midazolam-saline, and (4) midazolam-morphine groups. First, a saline or midazolam injection was given intraperitoneally and after 30 min a second injection of saline or morphine was given subcutaneously once daily for 11 days. Animals were sacrificed on the 11th day 60 min after the last injection to measure met-enkephalin by radioimmunoassay. Morphine tolerant animals showed a significant increase in met-enkephalin levels in the cortex (137%) and midbrain (89%), and a significant decrease in met-enkephalin levels in the pituitary (74%), cerebellum (34%) and medulla (72%). Midazolam treated animals showed a significant decrease in met-enkephalin levels in the pituitary (63%), cortex (39%), medulla (58%), kidneys (36%), heart (36%) and adrenals (43%), and a significant increase in met-enkephalin level...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A M Graybiel, M F Chesselet

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Citations

Mar 3, 2005·Pharmacology, Biochemistry, and Behavior·Jun-Li CaoYin-Ming Zeng
Jan 8, 1999·Peptides·G A OlsonA J Kastin
May 25, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lauren FagetDominique Massotte
Jan 31, 2006·Journal of Pain and Symptom Management·Alfredo H NaviganteMaria E Cabalar
Apr 16, 2013·Analytical Chemistry·Christian K FreseA F Maarten Altelaar
Nov 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Mikhail BoudarineYemiliya Berman

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