Met-Gly-Cys motif from G-protein alpha subunit cannot direct palmitoylation when fused to heterologous protein

Archives of Biochemistry and Biophysics
Toshihiko UtsumiH Iwata

Abstract

To determine whether the N-terminal Met-Gly-Cys motif from G-protein alpha subunit can direct palmitoylation of protein, we have generated heterologous fusion proteins containing the first 10 amino acids of Gi1 alpha and Gs alpha using tumor necrosis factor as a model protein and determined their ability to incorporate palmitate using in vitro and in vivo expression systems. DNA sequences coding for the N-terminal 10 amino acids of Gi1 alpha and Gs alpha were fused to the 5'-end of the cDNA coding for the mature domain of tumor necrosis factor (TNF) to give Gi1 alpha-TNF and Gs alpha-TNF cDNA. In vitro translation of the mRNA coding for the Gi1 alpha-TNF cDNA gave rise to an N-myristoylated fusion TNF with a molecular mass of 18 kDa as determined by the incorporation of [3H]myristic acid and by immunoprecipitation with anti-TNF antibody. In contrast, no incorporation of fatty acid was detected for Gs alpha-TNF. Baculovirus expression of the Gi1 alpha-TNF cDNA in Sf-9 cells gave rise to an N-myristoylated but not palmitoylated fusion TNF. This myristoylation was inhibited by replacement of Gly-2 with Ala but not Cys-3 with Ala, indicating the acylation reaction is entirely dependent on the N-myristoylation signal (Met-Gly-X-X-X-...Continue Reading

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Citations

Jul 4, 2001·FEBS Letters·T UtsumiR Ishisaka
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier

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