PMID: 8600369Mar 1, 1996Paper

Metabolic activation of benzo[a]pyrene in two fetal mouse hepatocyte lines: induction of DNA adducts and micronuclei

Mutation Research
S CanovaL Celotti

Abstract

We have studied the metabolic competence of two non-transformed epithelial-like cell lines derived from fetal mouse liver, C 6 and C 2.8, to activate the promutagen benzo[a]pyrene by measuring both the induction of DNA adducts through the nuclease P1-enhanced 32P-postlabeling assay and the formation of micronuclei. The pattern and level of DNA adducts detected in C 6 and C 2.8 cells treated with benzo[a]pyrene were compared with those obtained in human peripheral blood lymphocytes treated with the same compound and with [3H]anti-benzo[a]pyrene diolepoxide. In both the cell lines and in human lymphocytes we observed a consistent induction of distinct DNA adducts. In C 6 and C 2.8 cells, the most evident adduct showed a position similar to that of the main adduct induced by [3H]-anti-benzo[a]pyrene diolepoxide in human lymphocytes. In addition, benzo[a]pyrene caused a significant increase of micronucleated C 6 and C 2.8 cells, whereas the frequency of micronuclei did not increase in CHO cells treated, for comparison, in the same way.

References

Oct 1, 1993·Chemico-biological Interactions·T M Penning

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Citations

Dec 5, 1998·Chemosphere·C UraniM Camatini
Apr 24, 2010·Radiation Protection Dosimetry·Lawrence T DauerPhung Tran
Jan 1, 1997·Environmental and Molecular Mutagenesis·B van AgenJ C Kleinjans
Aug 8, 1997·The Journal of Biological Chemistry·K B Beckman, B N Ames
Mar 29, 2020·International Journal of Environmental Research and Public Health·Luigi VimercatiGiovanni Maria Ferri

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