Metabolic adaptation and oxaloacetate homeostasis in P. fluorescens exposed to aluminum toxicity

Journal of Basic Microbiology
Joseph LemireV D Appanna

Abstract

Microbial systems are known to elaborate intricate metabolic strategies in an effort to fend the toxic impact of numerous metals. In this study, we show that the exposure of Pseudomonas fluorescens to aluminum (Al) resulted in a metabolic shift aimed at diverting oxaloacetate towards the biogenesis of an aluminophore. This metabolic alteration was characterized by uncoupling of two gluconeogenic enzymes, namely pyruvate carboxylase (PC) and phosphoenolpyruvate carboxykinase (PEPCK). While PC displayed a sharp increase in activity and expression, PEPCK was severely diminished. Malic enzyme (ME) and NAD kinase (NADK), two enzymes involved in maintaining a reductive environment, were markedly increased in the Al-stressed cells. Hence, Al-exposed Pseudomonas fluorescens evoked a metabolic response aimed at generating oxaloacetate and promoting an intracellular reductive environment.

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Citations

Aug 13, 2011·Journal of Hazardous Materials·Elizabeth Lira-SilvaRicardo Jasso-Chávez
May 13, 2011·Journal of Proteome Research·Sean C BoothAalim M Weljie
Sep 17, 2011·Metallomics : Integrated Biometal Science·Sean C BoothRaymond J Turner
May 12, 2009·Acta Biochimica Et Biophysica Sinica·Feng ShiXiaoyuan Wang

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