Metabolic adaptations during extreme anoxia in the turtle heart and their implications for ischemia-reperfusion injury.

Scientific Reports
Amanda BundgaardAngela Fago

Abstract

ATP depletion and succinate accumulation during ischemia lead to oxidative damage to mammalian organs upon reperfusion. In contrast, freshwater turtles survive weeks of anoxia at low temperatures without suffering from oxidative damage upon reoxygenation, but the mechanisms are unclear. To determine how turtles survive prolonged anoxia, we measured ~80 metabolites in hearts from cold-acclimated (5 °C) turtles exposed to 9 days anoxia and compared the results with those for normoxic turtles (25 °C) and mouse hearts exposed to 30 min of ischemia. In turtles, ATP and ADP decreased to new steady-state levels during fasting and cold-acclimation and further with anoxia, but disappeared within 30 min of ischemia in mouse hearts. High NADH/NAD+ ratios were associated with succinate accumulation in both anoxic turtles and ischemic mouse hearts. However, succinate concentrations and succinate/fumarate ratios were lower in turtle than in mouse heart, limiting the driving force for production of reactive oxygen species (ROS) upon reoxygenation in turtles. Furthermore, we show production of ROS from succinate is prevented by re-synthesis of ATP from ADP. Thus, maintenance of an ATP/ADP pool and low succinate accumulation likely protects tur...Continue Reading

References

Dec 1, 1975·Circulation Research·M J RovettoJ R Neely
Mar 1, 1979·Journal of Molecular and Cellular Cardiology·S Mochizuki, J R Neely
Feb 21, 1975·Science·P W Hochachka, K B Storey
Jan 15, 1975·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P W HochachkaG C Whittow
Jan 17, 1986·Science·P W Hochachka
Jan 1, 1989·The American Journal of Physiology·A J Hulbert, P L Else
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·G R Ultsch
Nov 1, 1988·The American Journal of Physiology·D A Kelly, K B Storey
Jan 1, 1985·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·P L Else, A J Hulbert
Jan 1, 1981·The American Journal of Physiology·P L Else, A J Hulbert
Mar 15, 1980·The Biochemical Journal·H TaegtmeyerH A Krebs
Feb 1, 1980·Cardiovascular Research·A J DrakeM I Noble
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P W HochachkaS C Land
May 1, 1997·Molecular and Cellular Biochemistry·W G Willmore, K B Storey
Jan 5, 1999·European Journal of Biochemistry·C DepréL Hue
May 5, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·D C Jackson
Sep 16, 2000·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·R G Boutilier, J St-Pierre
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L T Buck
Jan 17, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tammie BishopMartin D Brand
Mar 1, 1957·The American Journal of Physiology·G L RAPATZ, X J MUSACCHIA
May 7, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Göran E Nilsson, Peter L Lutz
Nov 24, 2004·Respiratory Physiology & Neurobiology·Donald C Jackson
Feb 8, 2006·Annual Review of Physiology·Tobias WangDavid J Randall
May 17, 2006·Biological Reviews of the Cambridge Philosophical Society·Gordon R Ultsch
Jul 11, 2006·Physiological and Biochemical Zoology : PBZ·Daniel E WarrenDonald C Jackson
Oct 14, 2006·Annual Review of Physiology·Philip E Bickler, Leslie T Buck
Jul 24, 2007·Zoology : Analysis of Complex Systems, ZACS·Marshall D McCue
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Jul 10, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jonathan A W StecykHans-O Pörtner
Aug 9, 2013·The Journal of Experimental Biology·Gina L J GalliJeffrey G Richards
Oct 22, 2013·The Journal of Experimental Biology·Frank B JensenTobias Wang
Jan 19, 2016·Cell Metabolism·Edward T ChouchaniMichael P Murphy

❮ Previous
Next ❯

Citations

May 13, 2020·The Journal of Experimental Biology·Amanda BundgaardAngela Fago
Aug 14, 2019·Aging·Matthew E Pamenter, Daniel Munro
Sep 12, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anita DittrichHenrik Lauridsen
May 13, 2020·Nature Metabolism·Jack L MartinMichael P Murphy
Sep 5, 2020·Scientific Reports·Sergei V ShekhovtsovDaniil I Berman
May 22, 2019·The Journal of Experimental Biology·Birgitte JensenAngela Fago
Jul 12, 2020·The Journal of Experimental Biology·Georgina K Cox, Todd E Gillis
Sep 16, 2020·Laboratory Investigation; a Journal of Technical Methods and Pathology·Cheng-Long ZhangFei Li
Dec 5, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amanda BundgaardAngela Fago
Mar 20, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Alexander G LittleChristopher D Moyes
Mar 21, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alexander G LittleChristopher D Moyes
Mar 28, 2021·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Sarah A BreedonKenneth B Storey
Mar 30, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Alexander G LittleChristopher D Moyes
May 26, 2021·The Science of the Total Environment·Abraão Tiago Batista Guimarães, Guilherme Malafaia
Jun 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael O IseiCollins Kamunde
Aug 27, 2021·Metabolites·Alexander Myrka, Leslie Buck

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay

Software Mentioned

MetImp
R package muma
script
R

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.