Metabolic alterations in the chronically denervated dog heart

Cardiovascular Research
G J van der VusseM I Noble

Abstract

Previous studies have shown that chronic cardiac denervation impairs myocardial glucose oxidation. To investigate this further we tested whether the tissue content of glucose transporters, activity of glycolytic enzymes or metabolic capacity of pyruvate dehydrogenase were altered. Moreover, we investigated whether the decline in glucose utilization was associated with an upregulation of proteins and enzymes involved in fatty acid handling. Chronic cardiac denervation results also in decreased left ventricular efficiency. We explored whether alterations in mitochondrial properties could be held responsible for this phenomenon. Twelve adult dogs were included in the study. In 6 of them chronic cardiac denervation was accomplished by surgical ablation of the extrinsic nerve fibers. The other 6 dogs were sham-operated. Biopsies were obtained from the left ventricle after 4-5 weeks of denervation. The content or enzymatic activity of proteins involved in fatty acid and glucose handling was assessed. Features of glutamate oxidation were measured in freshly isolated mitochondria. The content or activity of a set of fatty acid handling proteins did not change during chronic cardiac denervation. In contrast GLUT1 content significantly i...Continue Reading

Citations

May 2, 2002·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·William T EvanochkoGerald M Pohost
May 31, 2002·Autonomic Neuroscience : Basic & Clinical·Jacques R RouleauJohn G Kingma
Sep 12, 2012·The Journal of Physiology·Claudio VimercatiFabio A Recchia
Jul 15, 2017·World Journal of Cardiology·John G KingmaJacques R Rouleau

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