Metabolic alterations in urine extracellular vesicles are associated to prostate cancer pathogenesis and progression

Journal of Extracellular Vesicles
Marc Clos-GarciaJuan M Falcón-Pérez

Abstract

Urine contains extracellular vesicles (EVs) that concentrate molecules and protect them from degradation. Thus, isolation and characterisation of urinary EVs could increase the efficiency of biomarker discovery. We have previously identified proteins and RNAs with differential abundance in urinary EVs from prostate cancer (PCa) patients compared to benign prostate hyperplasia (BPH). Here, we focused on the analysis of the metabolites contained in urinary EVs collected from patients with PCa and BPH. Targeted metabolomics analysis of EVs was performed by ultra-high-performance liquid chromatography-mass spectrometry. The correlation between metabolites and clinical parameters was studied, and metabolites with differential abundance in PCa urinary EVs were detected and mapped into cellular pathways. We detected 248 metabolites belonging to different chemical families including amino acids and various lipid species. Among these metabolites, 76 exhibited significant differential abundance between PCa and BPH. Interestingly, urine EVs recapitulated many of the metabolic alterations reported in PCa, including phosphathidylcholines, acyl carnitines, citrate and kynurenine. Importantly, we found elevated levels of the steroid hormone, ...Continue Reading

References

Apr 15, 1994·International Journal of Cancer. Journal International Du Cancer·A A ChaudryL E Moffat
Sep 27, 2003·BJU International·F H FaasD E Johnson
Jul 2, 2004·Nature Reviews. Cancer·Julian L Griffin, John P Shockcor
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Alisdair R FernieLothar Willmitzer
May 11, 2005·Cancer Letters·Kasem NithipatikomWilliam B Campbell
Dec 21, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Carmen RodriguezEugenia E Calle
May 27, 2011·The New England Journal of Medicine·Johann S de BonoUNKNOWN COU-AA-301 Investigators
Aug 17, 2012·International Journal of Oncology·Peiying YangJeri Kim
Oct 2, 2012·The American Journal of Pathology·Dolores Di VizioMichael R Freeman
Nov 10, 2012·Metabolomics : Official Journal of the Metabolomic Society·Esperanza GonzalezJuan M Falcon-Perez
Dec 6, 2012·Current Medicinal Chemistry·M S MonteiroP Guedes de Pinho
Jan 8, 2013·Advances in Cancer Research·Sahar A Saddoughi, Besim Ogretmen
Mar 1, 2013·Nature Reviews. Cancer·Arkaitz CarracedoPier Paolo Pandolfi
Mar 19, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maite Martínez-UñaJosé M Mato
Apr 9, 2013·Biochimica Et Biophysica Acta·Giorgia ZadraMassimo Loda
Nov 12, 2013·The Journal of Urology·Siebren DijkstraJack A Schalken
Aug 1, 2008·Future Lipidology·Kasem Nithipatikom, William B Campbell
May 17, 2015·Journal of Extracellular Vesicles·María Yáñez-MóOlivier De Wever
Jul 22, 2015·Nature Reviews. Gastroenterology & Hepatology·Elaine HolmesJeremy K Nicholson
Dec 4, 2015·British Journal of Cancer·Guro F GiskeødegårdMay-Britt Tessem
Mar 13, 2016·Hormones & Cancer·Cameron P CapperRichard J Auchus
Mar 22, 2016·Biomedical Reports·Srinivas PentyalaSardar Khan
May 24, 2016·Nature Cell Biology·Veronica TorranoArkaitz Carracedo
Jul 2, 2016·Current Opinion in Pharmacology·Veronica TorranoArkaitz Carracedo
Jul 8, 2016·International Journal of Molecular Sciences·Gagan Deep, Isabel R Schlaepfer
Aug 29, 2016·Translational Oncology·Ana Rita LimaPaula Guedes de Pinho
Oct 19, 2016·The Journal of Pathology·Daniel Nava RodriguesJohann de Bono
Nov 5, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Felix RoyoJuan M Falcon-Perez
Dec 4, 2016·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Tore SkotlandAlicia Llorente
Jan 31, 2017·Gastroenterology·Cristina AlonsoJosé M Mato
Apr 16, 2017·Molecular Cancer·Ashley Di MeoGeorge M Yousef
May 30, 2017·The Journal of Urology·Daniel J LeeDaniel A Barocas
Jun 29, 2017·Nature·Amaia Zabala-LetonaArkaitz Carracedo
Jul 4, 2017·Nature Chemical Biology·Nunzio IraciStefano Pluchino

❮ Previous
Next ❯

Citations

Dec 13, 2019·Nature Reviews. Urology·Johannes Linxweiler, Kerstin Junker
Sep 23, 2018·Biochemical Society Transactions·Sai V ChittiSuresh Mathivanan
Jan 31, 2019·Diagnostics·Jochen Neuhaus, Bo Yang
Jul 25, 2019·International Journal of Molecular Sciences·Aneta ZebrowskaMonika Pietrowska
Apr 23, 2019·Journal of Extracellular Vesicles·Mari PalviainenPia R-M Siljander
Jul 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Beatriz Martín-GraciaMaría Moros
Mar 16, 2019·Experimental & Molecular Medicine·András GézsiEdit I Buzás
Apr 9, 2020·Frontiers in Oncology·Giuseppina ComitoPaolo Cirri
Nov 14, 2019·Metabolites·Charles WilliamsJuan M Falcón-Pérez
Mar 13, 2019·Metabolites·Nuria Gómez-CebriánLeonor Puchades-Carrasco
Feb 23, 2019·Diagnostics·Marouane KdadraEric Schiffer
Jan 19, 2021·Seminars in Cancer Biology·Leanne L LeungKatie Meehan
Nov 11, 2019·Journal of Pharmaceutical and Biomedical Analysis·Malena ManziMaría Eugenia Monge
Jan 30, 2021·BioMed Research International·Yuan YaoXinwei Han
Nov 5, 2020·Journal of Extracellular Vesicles·Carolina SoekmadjiLei Zheng
Nov 12, 2020·Cancers·Mari PalviainenPia Siljander
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Zhengyu ZhangZizhong Hu
May 22, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Michael P RimmerRod T Mitchell
May 27, 2021·Journal of Extracellular Vesicles·Uta ErdbrüggerElena S Martens-Uzunova
Jun 5, 2021·Advanced Drug Delivery Reviews·Ana ŠpilakChrista Noehammer
Jun 18, 2021·Nature Protocols·Guillermo Bordanaba-FloritJuan M Falcón-Pérez

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA

Software Mentioned

Exog
dbWalk
ggplot2 R package
MetScape
stats
Cytoscape
SIMCA
OptimalCutpoints
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.