Metabolic analysis of Corynebacterium glutamicum during lactate and succinate productions under oxygen deprivation conditions

Journal of Molecular Microbiology and Biotechnology
Masayuki InuiHideaki Yukawa

Abstract

Lactate and succinate were produced from glucose by Corynebacterium glutamicum under oxygen deprivation conditions without growth. Addition of bicarbonate to the reaction mixture led not only to a 3.6-fold increase in succinate production rate, but also to a 2.3- and 2.5-fold increase, respectively, of the rates of lactate production and glucose consumption, compared to the control. Furthermore, when small amounts of pyruvate were added to the reaction mixture, acid production rates and the glucose consumption rate were multiplied by a factor ranging from 2 to 3. These phenomena were paralleled by an increase in the NAD(+)/NADH ratio, thus corroborating the view that the efficient regeneration of NAD(+) could be triggered by the addition of either bicarbonate or pyruvate. To investigate the global metabolism of corynebacteria under oxygen deprivation conditions, we engineered several strains where the genes coding for key metabolic enzymes had been inactivated by gene disruption and replacement. A lactate dehydrogenase (LDH)-deficient mutant was not able to produce lactate, suggesting this enzyme has no other isozyme. Although a pyruvate carboxylase (pyc) mutant exhibited similar behavior to that of the wild type, phosphoenolpy...Continue Reading

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Citations

Jan 27, 2005·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
Apr 22, 2005·Applied Microbiology and Biotechnology·Nobuaki SuzukiHideaki Yukawa
Apr 28, 2006·Applied Microbiology and Biotechnology·Juri M SeletzkyJochen Büchs
Jan 12, 2007·Applied Microbiology and Biotechnology·Toshihiro TatenoAkihiko Kondo
Mar 10, 2007·Applied Microbiology and Biotechnology·Taku NishimuraHideaki Yukawa
Jul 5, 2007·Applied Microbiology and Biotechnology·James B McKinlayJ Gregory Zeikus
Oct 3, 2007·Applied Microbiology and Biotechnology·Kaori YasudaHideaki Yukawa
Oct 30, 2007·Applied Microbiology and Biotechnology·Hideo KawaguchiHideaki Yukawa
Jan 12, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
Sep 9, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa
Oct 22, 2008·Applied Microbiology and Biotechnology·Yoshiki TsuchidaHideaki Yukawa
Dec 11, 2008·Applied Microbiology and Biotechnology·Nobuaki SuzukiHideaki Yukawa
Jun 17, 2009·Applied Microbiology and Biotechnology·Miho SasakiHideaki Yukawa
Oct 20, 2009·Applied Microbiology and Biotechnology·Toru JojimaHideaki Yukawa
Dec 17, 2009·Applied Microbiology and Biotechnology·Miho SasakiHideaki Yukawa
Sep 3, 2010·Applied Microbiology and Biotechnology·Stephanie HuhnKay Marin
Feb 10, 2011·Applied Microbiology and Biotechnology·Takeyuki TsuchidateAkihiko Kondo
Feb 18, 2011·Applied Microbiology and Biotechnology·Shogo YamamotoHideaki Yukawa
Apr 28, 2011·Applied Microbiology and Biotechnology·Hiroshi TeramotoHideaki Yukawa
Nov 19, 2011·Applied Microbiology and Biotechnology·Vipin GopinathK Madhavan Nampoothiri
Dec 1, 2011·Applied Microbiology and Biotechnology·Yuyang SongSeiichi Taguchi
Jan 10, 2012·Applied Microbiology and Biotechnology·Stefan WieschalkaBernhard J Eikmanns
Jan 12, 2013·Applied Microbiology and Biotechnology·Takeshi KubotaHideaki Yukawa
May 25, 2013·Applied Microbiology and Biotechnology·Yukihiro KitadeHideaki Yukawa
Oct 31, 2013·Applied Microbiology and Biotechnology·Verena Bückle-VallantBernhard J Eikmanns
Oct 2, 2009·Bioinformatics·Luis F de FigueiredoFrancisco J Planes
Apr 21, 2009·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Jul 1, 2008·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Jul 6, 2010·Applied and Environmental Microbiology·Haruhiko TeramotoHideaki Yukawa
Dec 18, 2012·Applied and Environmental Microbiology·Satoshi HasegawaHideaki Yukawa
Apr 7, 2009·Applied and Environmental Microbiology·Hideo KawaguchiHideaki Yukawa

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