Metabolic analysis of the rabbit cornea by proton nuclear magnetic resonance spectroscopy

Ophthalmic Research
A MidelfartI S Gribbestad

Abstract

Perchloric acid extracts of the rabbit cornea were analyzed by high-resolution 1H NMR spectroscopy, including the two-dimensional shift-correlated (COSY) technique. Numerous metabolites including leucine/isoleucine, 3-hydroxybutyrate, valine, lactate, alanine, lysine, acetate, glutamate, creatine/phosphocreatine, taurine, glycine, inositol, glucose, tyrosine, phenylalanine, histidine, adenosine and formate have been simultaneously detected and identified in the NMR spectra. Concentrations of some of these were also determined. Of particular importance are the detection and identification of hypotaurine in the corneal tissue. Hypotaurine is a precursor of the synthesis of taurine and may act as an antioxidant. Moreover, the presence of choline, a precursor for acetylcholine, was established. This novel metabolite profile of the cornea obtained by high-resolution 1H NMR spectroscopy may be of value in the study of pathologic and toxic disorders.

Citations

Apr 6, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Miroslav FrisAnna Midelfart
Feb 17, 2010·Journal of Ocular Biology, Diseases, and Informatics·Stephen P Young, Graham R Wallace
Jan 19, 2013·Current Eye Research·Tomasz KryczkaAnna Midelfart
Jul 17, 2009·Acta Ophthalmologica·Anna Midelfart
Nov 25, 2011·Acta Ophthalmologica·Tomasz KryczkaAnna Midelfart
Sep 22, 2011·Acta Ophthalmologica·Tomasz KryczkaAnna Midelfart
Jan 7, 2006·Acta Ophthalmologica Scandinavica·Anna Midelfart
May 17, 2011·The Journal of Eukaryotic Microbiology·Stephenson W NkininEdna S Kaneshiro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Forebrain- Circuits

Basal forebrain is a region in the brain important for production of acetylcholine and is the major cholinergic output of the CNS. Discover the latest research on circuits in the basal forebrain here.

Related Papers

Experimental Eye Research
J V GreinerT Glonek
International Ophthalmology
Samar A Al-Swailem
Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
K HayashiK R Kenyon
© 2022 Meta ULC. All rights reserved