Metabolic and gene expression hallmarks of seed germination uncovered by sodium butyrate in Medicago truncatula

Plant, Cell & Environment
Andrea PaganoAlma Balestrazzi

Abstract

Because high-quality seeds are essential for successful crop production in challenging environments, understanding the molecular bases of seed vigour will lead to advances in seed technology. Histone deacetylase inhibitors, promoting histone hyperacetylation, are used as tools to explore aspects still uncovered of the abiotic stress response in plants. The aim of this work was to investigate novel signatures of seed germination in Medicago truncatula, using the histone deacetylase inhibitor sodium butyrate (NaB) as stress agent. NaB-treated and untreated seeds collected at 2 and 8 hr of imbibition and at the radicle protrusion stage underwent molecular phenotyping and nontargeted metabolome profiling. Quantitative enrichment analysis revealed the influence of NaB on seed nucleotide, amino acid, lipid, and carbohydrate metabolism. Up-regulation of antioxidant and polyamine biosynthesis genes occurred in response to NaB. DNA damage evidenced in NaB-treated seeds correlated with up-regulation of base-excision repair genes. Changes in N1 -methyladenosine and N1 -methylguanine were associated with up-regulation of MtALKBH1 (alkylation repair homolog) gene. N2 ,N2 -dimethylguanosine and 5-methylcytidine, tRNA modifications involved i...Continue Reading

References

Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John D Storey, Robert Tibshirani
Dec 18, 2007·Molecular Cell·Ulrike BegleyThomas J Begley
Oct 22, 2008·BMC Research Notes·Luis Oñate-Sánchez, Jesús Vicente-Carbajosa
Jun 30, 2010·The Plant Journal : for Cell and Molecular Biology·Wanda M WaterworthChristopher E West
Feb 1, 2012·PloS One·Damian MieleckiElżbieta Grzesiuk
Apr 26, 2012·Nucleic Acids Research·Trine J MezaPål Ø Falnes
Mar 4, 2014·Chemical Reviews·Guanqun ZhengChuan He
Oct 2, 2014·Plant Physiology and Biochemistry : PPB·Zhi WangYongxiu Liu
Mar 31, 2015·Plant Cell Reports·S PaparellaAlma Balestrazzi
May 15, 2015·Frontiers in Plant Science·Marc Galland, Loïc Rajjou
Nov 12, 2015·Frontiers in Plant Science·Vasilissa Manova, Damian Gruszka
Dec 25, 2015·Journal of Integrative Plant Biology·Maria Elisa SabatiniAlma Balestrazzi
Feb 13, 2016·Frontiers in Oncology·Smrithi S MenonAnatoly Dritschilo
Jun 1, 2016·Frontiers in Plant Science·Susana de Sousa AraújoAlma Balestrazzi
Aug 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Wanda M WaterworthChristopher E West
Sep 8, 2016·Current Protocols in Bioinformatics·Jianguo Xia, David S Wishart
May 24, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fan YingEva Hoi Ching Tang
Jun 22, 2017·Plant Physiology·Adam D FeenstraYoung Jin Lee

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Citations

Dec 24, 2018·Plant, Cell & Environment·Christine H FoyerHon-Ming Lam
Nov 22, 2019·Frontiers in Plant Science·Ana Bernardina MenéndezOscar Adolfo Ruiz
Jun 13, 2020·Horticulture Research·Chiara FortiAlma Balestrazzi
Jun 20, 2019·Frontiers in Plant Science·Wanda M WaterworthChristopher E West
Nov 14, 2020·International Journal of Molecular Sciences·Myoung Hui LeeSuk Weon Kim

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