Metabolic and lipidomic investigation of the antiproliferative effects of coronatine against human melanoma cells

Scientific Reports
Hye-Youn KimHyung-Kyoon Choi

Abstract

Melanoma is the most aggressive form of skin cancer, with metastatic melanoma being refractory to currently available conventional therapies. In this study, we evaluated the inhibitory effect of coronatine (COR) on the proliferation of metastatic melanoma cells. COR inhibited the proliferation of melanoma cells but negligibly affected the proliferation of normal melanocytes. Comparative metabolic and lipidomic profiling using gas chromatography-mass spectrometry and direct infusion-mass spectrometry was performed to investigate COR-induced metabolic changes. These analyses identified 33 metabolites and 82 lipids. Of these, the levels of lactic acid and glutamic acid, which are involved in energy metabolism, significantly decreased in COR-treated melanoma cells. Lipidomic profiling indicated that ceramide levels increased in COR-treated melanoma cells, suggesting that ceramides could function as a suppressor of cancer cell proliferation. In contrast, the levels of phosphatidylinositol (PI) species, including PI 16:0/18:0, 16:0/18:1, 18:0/18:0, and 18:0/18:1, which were found to be potential biomarkers of melanoma metastasis in our previous study, were lower in the COR-treated cells than in control cells. The findings of metabolo...Continue Reading

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Citations

Apr 21, 2020·Metabolomics : Official Journal of the Metabolomic Society·Jeremy P KoelmelJohn A Bowden
Jul 10, 2021·Progress in Lipid Research·Do-Hyeon KimSung Ho Ryu
May 14, 2020·Journal of Proteome Research·Ryan EghlimiHaiwei Gu

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Methods Mentioned

BETA
surgical resection
transgenic
enzyme-linked immunosorbent assay
glucosylation
Protein Assay
chip
PCA

Software Mentioned

Excel
OPLS
MetaboAnalyst
SPSS
Expressionist ® MSX
CompuSyn
ProteoWizard msConvert
DA
SIMCA
Xcalibur

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