Metabolic and permeability changes caused by thiamine deficiency in immortalized rat brain microvessel endothelial cells

Brain Research
I A RomeroN J Abbott

Abstract

The possible involvement of blood-brain barrier (BBB) breakdown in the pathogenesis of thiamine deficiency encephalopathy was investigated in RBE4 cells, an immortalized rat brain endothelial cell line. The effects of thiamine deficiency produced by addition of pyrithiamine and by reduction of thiamine in the culture medium, on the metabolism and permeability of the RBE4 monolayer was examined. Pyrithiamine treatment in low thiamine medium (M199) for 7 days caused cytotoxic effects on RBE4 cells at all concentrations (10-50 microg/ml). Pyrithiamine caused a concentration- and time-dependent decrease in MTT reduction and a significant increase in glucose consumption and lactate production compared to controls. Pyrithiamine treatment for 3 days caused a significant decrease in MTT reduction at 50 microg/ml only. In contrast, increased glucose consumption and lactate production by the RBE4 cells was observed after treatment for 3 days with concentrations of 25 microg/ml pyrithiamine and above. The permeability of RBE4 cell monolayers to [14C]sucrose (Mw 342), but not FITC-dextran (Mw 4000) was significantly increased by treatment with pyrithiamine concentrations of 25 microg/ml and above for 3 days. These effects were not accompan...Continue Reading

Citations

Sep 6, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B B WekslerP O Couraud
Jun 27, 2013·Pharmaceutical Research·Céline Passeleu-Le BourdonnecSophie Martel
Jun 21, 2005·Cellular and Molecular Neurobiology·Françoise Roux, Pierre-Olivier Couraud
Jun 21, 2005·Cellular and Molecular Neurobiology·Máiria A DeliMasami Niwa
Apr 16, 2008·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·A González-Quevedo MonteagudoR Santiago Luis González
Apr 2, 2020·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Susana Alcázar-LeyvaNoé Alvarado-Vásquez
Apr 1, 2000·Journal of Neuropathology and Experimental Neurology·N Y CalingasanG E Gibson

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