Metabolic and taxonomic insights into the Gram-negative natural rubber degrading bacterium Steroidobacter cummioxidans sp. nov., strain 35Y

PloS One
Vikas SharmaTulika Prakash

Abstract

The pathway of rubber (poly [cis-1,4-isoprene]) catabolism is well documented for Gram-positive rubber degraders but only little information exists for Gram-negative species. The first documented potent rubber degrading Gram-negative strain is Xanthomonas sp. strain 35Y that uses extracellular rubber oxygenases for the initial cleavage of the polyisoprene molecule. However, neither the exact phylogenetic position of Xanthomonas sp. strain 35Y nor the catabolic pathway of the primary polyisoprene cleavage products have been investigated. In this contribution, we started to address both these issues by a comprehensive taxonomic characterization and by the analysis of the draft genome sequence of strain 35Y. Evaluation of the 16S rRNA gene sequence pointed to a borderline taxonomic position of strain 35Y as a novel species of the genus Steroidobacter. Further, substantial differences in the genotypic properties of strain 35Y and the members of the genus Steroidobacter, including average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH), resolved the taxonomic position of strain 35Y and suggested its positioning as a novel species of the genus Steroidobacter. This was further confirmed by comparative analysis of p...Continue Reading

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Nov 1, 2018·Applied Microbiology and Biotechnology·Dieter Jendrossek, Jakob Birke
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Aug 4, 2021·International Journal of Systematic and Evolutionary Microbiology·Rui-Rui HuangWen-Zheng Liu

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Datasets Mentioned

BETA
KC980911.1
KY498024.1

Methods Mentioned

BETA
Illumina sequencing
fluorescence microscopy
FACS

Software Mentioned

JSpecies
MIRA ( Mimicking Intelligent Read Assembly ) assembler
BioNJ
MEGA6
WebMGA
BLASTP
BLASTN
SecretomeP
CRISPRfinder
ISfinder

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