Abstract
Foreign protein production levels in two recombinant Chinese hamster ovary (CHO) cell lines were compared in cells transfected with different expression vectors. One vector pNL1 contained the gene for neomycin resistance (neo ( r )) and thelacZ gene which codes for intracellular β-galactosidase, with both genes controlled by the constitutive simian virus (SV40) promoter. The other vector CDβG contained the amplifiabledhfr gene andlacZ gene, controlled by the constitutive SV40 and cytomegalovirus (CMV) promoters, respectively. Cell growth and β-galactosidase expression were compared quantitatively after cells were selected in different concentrations of the neomycin analog G418 and methotrexate, respectively. A 62% reduction in growth rate occurred in recombinant CHO cells in which thelacZ anddhfr genes were highly amplified and expressed. In contrast, the combined effects of the unamplifiedneo ( r ) gene andlacZ gene expression on the growth kinetics were small. Any metabolic burden caused bylacZ gene expression, which was evaluated separately from the effect ofneo ( r ) gene expression, must be negligible, as higher expression of β-galactosidase (1.5×10(-6) units/cell) occurred in unamplified cells compared to the cells in whi...Continue Reading
References
Dec 8, 1978·Science·R T SchimkeR F Kellems
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·W HoeckB Groner
Jul 15, 1992·Biochemical and Biophysical Research Communications·M H RickettsB C Chung
Jan 1, 1992·Cytotechnology·M B GuD S Kompala
Nov 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G HuperJ D Iglehart
Jul 1, 1990·Biologicals : Journal of the International Association of Biological Standardization·F M Wurm
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M TakeuchiA Kobata
Jul 1, 1985·Molecular and Cellular Biology·R J KaufmanR M Kay
Jul 1, 1983·Molecular and Cellular Biology·W E Bradley
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S J ScahillW Fiers
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L R Beach, R D Palmiter
Jun 5, 1992·Biotechnology and Bioengineering·G J PendseJ E Bailey
Nov 5, 1993·Biotechnology and Bioengineering·M B GuD S Kompala
Citations
Apr 1, 1999·Biotechnology and Bioengineering·N S KimG M Lee
Apr 1, 1999·Biotechnology and Bioengineering·C H FannJ M Piret
Jan 1, 1996·Cytotechnology·G G BanikD S Kompala
Apr 25, 2012·Applied Microbiology and Biotechnology·Eugéne van RensburgJohann F Görgens
Nov 13, 2008·Cytotechnology·D R LloydM Al-Rubeai
Nov 13, 2008·Cytotechnology·C A Yallop, I Svendsen
Nov 13, 2008·Cytotechnology·Christel FengeMichael Wood
Nov 13, 2008·Cytotechnology·C A YallopI Svendsen
Oct 23, 2013·PloS One·Raihana Z EdrosMohamed Al-Rubeai
Mar 21, 2001·Biotechnology and Bioengineering·J F GörgensB Hahn-Hägerdal
Nov 4, 2008·Biotechnology and Bioengineering·Janet ChusainowMiranda G S Yap
Feb 23, 2008·Biotechnology Journal·Chaya MohanGyun Min Lee
Jun 20, 2015·Molecular Biotechnology·Zeynep Betts, Alan J Dickson
Feb 19, 2014·BMC Biotechnology·Raihana EdrosMohamed Al-Rubeai
Dec 7, 2016·Biotechnology and Bioengineering·Jong Youn Baik, Kelvin H Lee
Jul 7, 2017·Biotechnology and Applied Biochemistry·Wu ZouMohamed Al-Rubeai
Dec 21, 2017·Scientific Reports·Quang Vinh PhanManfred Gossen
Jan 24, 2019·Bioprocess and Biosystems Engineering·Kahina MellahiOlivier Henry
Feb 22, 2011·Biotechnology and Bioengineering·Britta KrampeMohamed Al-Rubeai
Jun 22, 2000·Biotechnology and Bioengineering·C H FannJ M Piret
May 9, 2019·Biotechnology Letters·N JiménezZ P Gerdtzen
Jan 4, 2020·Nature Communications·Jahir M GutierrezNathan E Lewis