PMID: 45556Jan 1, 1979

Metabolic changes in Crithidia fasciculata accompanying physiological adaptation to growth in the presence of carbonyl cyanide m-chlorophenylhydrazone

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
R S Kutzman, J F Roberts


1. Crithidia fasciculata adapted to growth in the presence of 10(-5) M carbonyl cyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, maintained adenosine phosphate pools and an adenylate energy charge comparable to those of control cells. 2. CCCP-adapted cells in the presence of the uncoupler respire endogenous substrate at a greater rate than control cells and this effect of CCCP appears readily reversible. 3. CCCP-treated, adapted cells, supporting high endogenous respiration rates, were not responsive to added substrates which significantly stimulated the oxygen utilization of normal C. fasciculata. 4. CCCP-adapted cells, provided with [U-14C]-labeled proline, utilize this substrate at 67% the rate of control cells, but divide the isotopic label between CO2 and protein in a ratio identical to that of normal cells. 5. The transport of alanine and proline by adapted C. fasciculata was severely impaired, while the transport of tyrosine and leucine was unaffected.


Jan 1, 1978·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·R S Kutzman, J F Roberts
Jan 1, 1973·Annual Review of Biochemistry·J E Casida
Mar 26, 1973·Archiv für Mikrobiologie·H Nitsche
May 1, 1974·The Journal of protozoology·M D Ruff, C P Read
Jan 1, 1969·Annual Review of Microbiology·D E Atkinson
Jun 1, 1969·Experimental Parasitology·S M Krassner
Oct 1, 1974·Plant Physiology·D E MorelandF S Farmer
Dec 1, 1977·Journal of Bioenergetics and Biomembranes·J S Kahn, R T McConnell

Related Concepts

Adaptation, Physiological
Adenine Nucleotides
Carbon Dioxide
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Energy Metabolism
Hydrogen-Ion Concentration

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