Metabolic characterization of human aqueous humor in the cataract progression after pars plana vitrectomy

BMC Ophthalmology
Yinghong JiYi Lu

Abstract

While pars plana vitrectomy (PPV) has become the third most commonly performed surgery in the world, it can also induce multiple post complications easily. Among them, cataract progression is the most frequent one that can lead to blindness eventually. To understand the underlying mechanisms of post PPV cataract progression, we performed comprehensive metabolic characterization of aqueous humor (AH) samples from 20 cataract patients (10 post PPV complication and 10 none PPV cataract) by a non-targeted metabolomic analysis using gas chromatography combined with time-of-flight mass spectrometer (GC/TOF MS). A total of 263 metabolites were identified and eight of them are determined to be significantly different (VIP ≥ 1 and p ≤ 0.05) between post PPV group and none PPV control group. The significantly changed metabolites included glutaric acid and pelargonic acid that play key roles in the regulation of oxidative stress and inflammatory responses. Furthermore, we constructed a metabolic regulatory network in each group based on metabolite-metabolite correlations, which reveals key metabolic pathways and regulatory elements including amino acids and lipids metabolisms that are related to cataract progression. Altogether, this work...Continue Reading

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Mar 23, 2017·Experimental Eye Research·Yinghong JiYi Lu

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Citations

Nov 30, 2019·The Tohoku Journal of Experimental Medicine·Chian-Feng ChenChing-Yao Tsai
Jul 28, 2019·Diabetes Therapy : Research, Treatment and Education of Diabetes and Related Disorders·Kang XiaoGuang-Ming Wan
May 10, 2019·Metabolomics : Official Journal of the Metabolomic Society·Emanuela LocciErnesto d'Aloja
Jul 3, 2021·Journal of Clinical Medicine·Enrique García-GenF Bosch-Morell
Feb 1, 2019·Journal of Proteome Research·Adrien BuissetJuan Manuel Chao de la Barca

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Methods Mentioned

BETA
NMR
nuclear
electrophoresis

Software Mentioned

Mev
PLS
SPSS
DA
MultiExperiment Viewer )
R

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