Metabolic control of respiratory levels in coenzyme Q biosynthesis-deficient Escherichia coli strains leading to fine-tune aerobic lactate fermentation

Biotechnology and Bioengineering
Hui WuKa-Yiu San

Abstract

A novel strategy to finely control the electron transfer chain (ETC) activity of Escherichia coli was established. In this study, the fine-tuning of the ubiquinone biosynthesis pathway was applied to further controlling ETC function in coenzyme Q8 biosynthesis-deficient E. coli strains, HW108 and HW109, which contain mutations in ubiE and ubiG, respectively. A competing pathway on the intermediate substrates of the Q8 synthesis pathway, catalyzed by diphosphate:4-hydroxybenzoate geranyltransferase (PGT-1) of Lithospermum erythrorhizon, was introduced into these mutant strains. A nearly theoretical yield of lactate production can be achieved under fully aerobic conditions via an in vivo, genetically fine-tunable means to further control the activity of the ETC of the Q8 biosynthesis-deficient E. coli strains.

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Citations

Mar 1, 2016·Biotechnology for Biofuels·Jianming LiuChristian Solem
Nov 2, 2016·Journal of Industrial Microbiology & Biotechnology·George N Bennett, Ka-Yiu San
Jul 12, 2019·Frontiers in Plant Science·Hiroaki KusanoKazufumi Yazaki
Nov 30, 2019·Frontiers in Bioengineering and Biotechnology·Uldis KalnenieksReinis Rutkis
Aug 31, 2020·Critical Reviews in Biotechnology·Zaiwei ManZhiqiang Cai

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