Metabolic disturbances in synovial fluid are involved in the onset of synovitis in heifers with acute ruminal acidosis

Scientific Reports
Pablo AlarcónRafael Agustín Burgos

Abstract

Acute ruminal acidosis (ARA) is the result of increased intake of highly fermentable carbohydrates, which frequently occurs in dairy cattle and is associated with aseptic polysynovitis. To characterise the metabolic changes in the joints of animals with ARA, we performed an untargeted gas chromatography-mass spectrometry (GC-MS)-based metabolomic analysis of synovial fluid. Seven heifers were challenged with an intraruminal oligofructose overload (13 g/kg of body weight [BW]) dissolved in water. Synovial fluid samples were collected at 0, 9 and 24 h post-overload. Metabolome analysis revealed the presence of 67 metabolites. At 9 h post-overload, glyceric acid, cellobiose, fructose and lactic acid were all increased, whereas at 24 h, sorbitol, lactic acid and fructose levels were all increased >10-fold. At 24 h, citric acid and threonine levels were significantly reduced. We detected increased L- and D-lactate, and the presence of interleukin-6 (IL-6) in synovial fluid. Furthermore, using bovine fibroblast-like synoviocytes, we observed that D-lactate induces IL-6 synthesis. Our results suggest that ARA produces severe metabolomic changes in synovial fluid, including disturbances in starch and sucrose metabolism, and increased l...Continue Reading

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Citations

May 7, 2020·Frontiers in Veterinary Science·Shuaichen LiJiantao Zhang
Jul 21, 2020·BioMed Research International·Miroslav Pohanka
Jan 5, 2021·Frontiers in Veterinary Science·Shuaichen LiNa Zhang

Methods Mentioned

BETA
PCA
ELISA
PCR

Related Concepts

Cellobiose
Fibroblasts
Food
Fructose
Interleukin-6
Joints
Gas Chromatography-Mass Spectrometry
Sorbitol
Starch
Synovial Fluid

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