Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production

Science China. Life Sciences
Shujuan LaiTingyi Wen

Abstract

L-Serine plays a critical role as a building block for cell growth, and thus it is difficult to achieve the direct fermentation of L-serine from glucose. In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and glycine, releasing the feedback inhibition by L-serine to 3-phosphoglycerate dehydrogenase (PGDH), in combination with the co-expression of 3-phosphoglycerate kinase (PGK) and feedback-resistant PGDH (PGDH(r)). The resulting strain, SER-8, exhibited a lower specific growth rate and significant differences in L-serine levels from Phase I to Phase V as determined for fed-batch fermentation. The intracellular L-serine pool reached (14.22 ± 1.41) μmol g(CDM) (-1), which was higher than glycine pool, contrary to fermentation with the wild-type strain. Furthermore, metabolic flux analysis demonstrated that the over-expression of PGK directed the flux of the pentose phosphate pathway (PPP) towards the glycolysis pathway (EMP), and the expression of PGDH(r) improved the L-serine biosynthesis pathway. In addition, the flux from L-serine to glycine dropped by 24%, indicating that the deletion of the activator GlyR resulted in down-regulation ...Continue Reading

References

Jul 1, 1993·Applied Microbiology and Biotechnology·Y IzumiT Tanabe
Apr 27, 2002·Applied and Environmental Microbiology·Eva RadmacherLothar Eggeling
Sep 1, 1964·Journal of Bacteriology·L I PIZER, M L POTOCHNY
Dec 3, 2003·The Journal of Biological Chemistry·Cristy L GellingIan W Dawes
Dec 3, 2004·Applied and Environmental Microbiology·Roman NetzerHermann Sahm
Nov 5, 2005·Applied and Environmental Microbiology·Petra Peters-WendischLothar Eggeling
Dec 24, 2005·Applied Microbiology and Biotechnology·Masato Ikeda
Jan 27, 2006·FEMS Microbiology Letters·Yutaka HaitaniHiroshi Takagi
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mee-Jung Han, Sang Yup Lee
Dec 5, 2006·Applied and Environmental Microbiology·Michael StolzLothar Eggeling
Jul 3, 2007·Metabolic Engineering·Soo-Dong ParkHeung-Shick Lee
Mar 15, 1993·Biotechnology and Bioengineering·J J Vallino, G Stephanopoulos
Nov 6, 2008·Biotechnology and Bioengineering·Kjeld Raunkjaer Kjeldsen, Jens Nielsen
Dec 17, 2008·Analytical Biochemistry·Hilal Taymaz-NikerelWalter M van Gulik
Jan 7, 2009·Journal of Biotechnology·Jens-Eric SchweitzerLothar Eggeling

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Citations

Mar 4, 2014·PLoS Computational Biology·Anupam ChowdhuryCostas D Maranas
Jul 7, 2014·Journal of Industrial Microbiology & Biotechnology·Pengfei GuQingsheng Qi
Sep 18, 2014·FEMS Microbiology Reviews·Víctor de LorenzoAntoine Danchin
Jan 13, 2016·Applied Microbiology and Biotechnology·Pengfei GuQingsheng Qi
Jan 21, 2015·Frontiers in Bioengineering and Biotechnology·Ali KhodayariCostas D Maranas
Dec 2, 2014·Applied Microbiology and Biotechnology·Qinjian ZhuZhenghong Xu
Aug 20, 2019·Journal of Industrial Microbiology & Biotechnology·Wei LiuZhinan Xu
Jul 7, 2017·Biotechnology for Biofuels·Yu ZhangTingyi Wen
Mar 15, 2020·Microbial Cell Factories·Chenyang WangZhijun Zhao
Dec 1, 2019·Journal of Industrial Microbiology & Biotechnology·Wei LiuZhinan Xu

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