Metabolic engineering for efficient supply of acetyl-CoA from different carbon sources in Escherichia coli

Microbial Cell Factories
Shasha ZhangYong Tao

Abstract

Acetyl-CoA is an important metabolic intermediate and serves as an acetylation precursor for the biosynthesis of various value-added acetyl-chemicals. Acetyl-CoA can be produced from glucose, acetate, or fatty acids via metabolic pathways in Escherichia coli. Although glucose is an efficient carbon source for acetyl-CoA production, the pathway from acetate to acetyl-CoA is the shortest and fatty acids can produce acetyl-CoA through fatty acid oxidation along with abundant NADH and FADH2. In this study, metabolically engineered E. coli strains for efficiently supplying acetyl-CoA from glucose, acetate, and fatty acid were constructed and applied in one-step biosynthesis of N-acetylglutamate (NAG) from glutamate and acetyl-CoA. A metabolically engineered E. coli strain for NAG production was constructed by overexpressing N-acetylglutamate synthase from Kitasatospora setae in E. coli BW25113 with argB and argA knockout. The strain was further engineered to utilize glucose, acetate, and fatty acid to produce acetyl-CoA. When glucose was used as a carbon source, the combined mutants of ∆ptsG::glk, ∆galR::zglf, ∆poxB::acs, ∆ldhA, and ∆pta were more efficient for supplying acetyl-CoA. The acetyl-CoA synthetase (ACS) pathway and acetat...Continue Reading

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Citations

Apr 29, 2020·Metabolites·Jason T KuEthan I Lan
Jan 30, 2020·Journal of Biological Research·Yujin CaoHuizhou Liu
Oct 20, 2020·Frontiers in Bioengineering and Biotechnology·Zhiquan WangYuanyuan Ma
Jan 12, 2021·Bioresource Technology·Virginia Montiel-Corona, Germán Buitrón
May 10, 2021·Trends in Biotechnology·Li ZhuSheng Yang
Aug 4, 2021·Nature Reviews. Microbiology·José Montaño LópezJosé L Avalos
Nov 2, 2021·ACS Synthetic Biology·Biaobiao LuoXuebo Hu
Nov 11, 2021·Journal of Agricultural and Food Chemistry·Qingxuan MuBo Yu

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Methods Mentioned

BETA
acetylation
genetic modification
PCR
transfection
acetylating

Software Mentioned

Generay

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