Metabolic engineering of an acid-tolerant yeast strain Pichia kudriavzevii for itaconic acid production

Metabolic Engineering Communications
Wan SunZengyi Shao

Abstract

Itaconic acid (IA), or 2-methylenesuccinic acid, has a broad spectrum of applications in the biopolymer industry owing to the presence of one vinyl bond and two acid groups in the structure. Its polymerization can follow a similar mechanism as acrylic acid but additional functionality can be incorporated into the extra beta acid group. Currently, the bio-based production of IA in industry relies on the fermentation of the filamentous fungus Aspergillus terreus. However, the difficulties associated with the fermentation undertaken by filamentous fungi together with the pathogenic potential of A. terreus pose a serious challenge for industrial-scale production. In recent years, there has been increasing interest in developing alternative production hosts for fermentation processes that are more homogenous in the production of organic acids. Pichia kudriavzevii is a non-conventional yeast with high acid tolerance to organic acids at low pH, which is a highly desirable trait by easing downstream processing. We introduced cis-aconitic acid decarboxylase gene (cad) from A. terreus (designated At_cad) into this yeast and established the initial titer of IA at 135 ​± ​5 ​mg/L. Subsequent overexpression of a native mitochondrial tricarb...Continue Reading

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Citations

Apr 25, 2020·Biotechnology Progress·Zia FatmaHuimin Zhao
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Jul 25, 2021·Current Opinion in Biotechnology·Shuobo ShiJens Nielsen
Oct 13, 2021·ACS Synthetic Biology·Andrew D McNaughtonNeeraj Kumar
Oct 26, 2021·Bioresource Technology·Hideo KawaguchiAkihiko Kondo

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Methods Mentioned

BETA
restriction
PCR
flow cytometry

Software Mentioned

Zeiss Zen Lite
CHOPCHOP
BD FACSCanto Clinical Software
Zeiss Zen Black

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