Metabolic engineering of Corynebacterium glutamicum ATCC13869 for L-valine production

Metabolic Engineering
Cheng ChenX Wang

Abstract

In this study, an L-valine-producing strain was developed from Corynebacterium glutamicum ATCC13869 through deletion of the three genes aceE, alaT and ilvA combined with the overexpression of six genes ilvB, ilvN, ilvC, lrp1, brnF and brnE. Overexpression of lrp1 alone increased L-valine production by 16-fold. Deletion of the aceE, alaT and ilvA increased L-valine production by 44-fold. Overexpression of the six genes ilvB, ilvN, ilvC, lrp1, brnE and brnF in the triple deletion mutant WCC003 further increased L-valine production. The strain WCC003/pJYW-4-ilvBNC1-lrp1-brnFE produced 243mM L-valine in flask cultivation and 437mM (51g/L) L-valine in fed-batch fermentation and lacked detectable amino-acid byproduct such as l-alanine and l-isoleucine that are usually found in the fermentation of L-valine-producing C. glutamicum.

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Citations

Jan 19, 2016·Enzyme and Microbial Technology·Guoqiang HanXiaoyuan Wang
Sep 14, 2015·Applied Microbiology and Biotechnology·Christopher M JonesBrian F Pfleger
Jul 29, 2015·Enzyme and Microbial Technology·Guoqiang HanXiaoyuan Wang
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Sep 1, 2016·Applied Microbiology and Biotechnology·Yadi LiuXiaoyuan Wang
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Mar 30, 2018·Applied Microbiology and Biotechnology·Xiaoyuan WangPeter J Quinn
Aug 7, 2017·MicrobiologyOpen·Yadi LiuXiaoyuan Wang
Jul 8, 2018·Biotechnology and Bioengineering·Adam W WestbrookC Perry Chou
Jun 1, 2020·Biotechnology and Applied Biochemistry·Yanchao ZhangXiaoyuan Wang
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Jan 29, 2020·Applied Microbiology and Biotechnology·Xinyu ZouZhibin Feng
Jun 15, 2020·Journal of Industrial Microbiology & Biotechnology·Ying-Yu WangWei-Guo Zhang
Apr 27, 2019·International Journal of Molecular Sciences·Ying-Yu WangLi-Ming Liu
Nov 18, 2018·Journal of Industrial Microbiology & Biotechnology·Milin ZhanYe Ni
Jul 25, 2018·World Journal of Microbiology & Biotechnology·Qin-Geng HuangJian-Zhong Huang
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
Apr 23, 2020·Frontiers in Bioengineering and Biotechnology·Dongbo CaiShouwen Chen
Sep 1, 2021·Microbial Cell Factories·Hui GaoZhiming Rao

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