Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin

Microbial Cell Factories
Judith BeckerChristoph Wittmann

Abstract

Cis, cis-muconic acid (MA) is a dicarboxylic acid of recognized industrial value. It provides direct access to adipic acid and terephthalic acid, prominent monomers of commercial plastics. In the present work, we engineered the soil bacterium Corynebacterium glutamicum into a stable genome-based cell factory for high-level production of bio-based MA from aromatics and lignin hydrolysates. The elimination of muconate cycloisomerase (catB) in the catechol branch of the β-ketoadipate pathway provided a mutant, which accumulated MA at 100% molar yield from catechol, phenol, and benzoic acid, using glucose as additional growth substrate. The production of MA was optimized by constitutive overexpression of catA, which increased the activity of the encoded catechol 1,2-dioxygenase, forming MA from catechol, tenfold. Intracellular levels of catechol were more than 30-fold lower than extracellular levels, minimizing toxicity, but still saturating the high affinity CatA enzyme. In a fed-batch process, the created strain C. glutamicum MA-2 accumulated 85 g L-1 MA from catechol in 60 h and achieved a maximum volumetric productivity of 2.4 g L-1 h-1. The strain was furthermore used to demonstrate the production of MA from lignin in a cascad...Continue Reading

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Citations

May 28, 2019·Journal of Industrial Microbiology & Biotechnology·Eri ShinodaEiji Masai
Jul 5, 2019·Journal of Industrial Microbiology & Biotechnology·Kirsten DavisLaura R Jarboe
Jul 30, 2020·Biotechnology and Applied Biochemistry·Mamata Singhvi, Beom Soo Kim
Aug 29, 2020·Biomolecules·Sisun ChoiEung-Soo Kim
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Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Bin ZhangXiao-Yu Wu
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Nov 18, 2021·Biotechnology and Bioengineering·Guokun WangIrina Borodina

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Methods Mentioned

BETA
PCR
genetic modification
Protein Assay

Software Mentioned

pClik
Sci
DASGIP
Ed
OriginLab
Clone Manager Professional

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