Metabolic engineering of Dunaliella salina for production of ketocarotenoids

Photosynthesis Research
N AnilaR Sarada

Abstract

Dunaliella is a commercially important marine alga producing high amount of β-carotene. The use of Dunaliella as a potential transgenic system for the production of recombinant proteins has been recently recognized. The present study reports for the first time the metabolic engineering of carotenoid biosynthesis in Dunaliella salina for ketocarotenoid production. The pathway modification included the introduction of a bkt gene from H. pluvialis encoding β-carotene ketolase (4,4'β-oxygenase) along with chloroplast targeting for the production of ketocarotenoids. The bkt under the control of Dunaliella Rubisco smaller subunit promoter along with its transit peptide sequence was introduced into the alga through standardized Agrobacterium-mediated transformation procedure. The selected transformants were confirmed using GFP and GUS expression, PCR and southern blot analysis. A notable upregulation of the endogenous hydroxylase level of transformants was observed where the BKT expression was higher in nutrient-limiting conditions. Carotenoid analysis of the transformants through HPLC and MS analysis showed the presence of astaxanthin and canthaxanthin with maximum content of 3.5 and 1.9 µg/g DW, respectively. The present study repor...Continue Reading

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Citations

Apr 21, 2018·Critical Reviews in Food Science and Nutrition·Ming-Hua LiangJian-Guo Jiang
Feb 2, 2019·Critical Reviews in Food Science and Nutrition·Dinesh Kumar SainiPratyoosh Shukla
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Gulshan KumarTilak Raj Sharma
Sep 13, 2020·Applied Microbiology and Biotechnology·Shuying FengYu Liu
Oct 30, 2019·Critical Reviews in Food Science and Nutrition·Zhi-Cong LiangJian-Guo Jiang
Jun 15, 2021·Applied Microbiology and Biotechnology·Peng-Wei HuangHe Huang
Oct 2, 2021·Journal of Agricultural and Food Chemistry·Nellie FrancezonLucie Beaulieu
Nov 28, 2021·World Journal of Microbiology & Biotechnology·Parminder Kaur NarangVishakha Raina

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