Metabolic engineering of E. coli for the production of O -succinyl-l-homoserine with high yield

3 Biotech
Jian-Feng HuangYu-Guo Zheng

Abstract

O-succinyl-l-homoserine (OSH) is a promising platform chemical for the production of C4 chemicals with huge market potential which can be produced by fermentation from glucose. To construct a strain capable of producing OSH with high yield, the metJ (encodes transcriptional repressor) and metI (encodes a subunit of dl-methionine transporter) were deleted in Escherichia coli W3110 to obtain a strain E. coli ∆JI. Then, overexpression of metL (encodes bifunctional aspartate kinase/homoserine dehydrogenase II) and inactivation of metB (encodes cystathionine γ-synthase) were implemented in one step, and the OSH titer of the resulting strain E. coli ∆JIB* TrcmetL was dramatically increased to 7.30 g/L. The feedback regulation was further relieved by progressively overexpressing metA fbr (encodes homoserine O-succinyltransferase), yjeH (encodes l-methionine exporter), and thrA fbr (encodes bifunctional aspartate kinase/homoserine dehydrogenase I) to increase the metabolic flux from aspartate to OSH. The 100% rationally designed strain E. coli ∆JIB* TrcmetL/pTrc-metA fbr -Trc-thrA fbr -yjeH produced 9.31 g/L OSH from 20 g/L glucose (0.466 g/g glucose) in batch fermentation, which represents the highest OSH yield from glucose report...Continue Reading

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Citations

Apr 17, 2020·Biotechnology Journal·Ying ZhuChun Li
Aug 18, 2020·Applied and Environmental Microbiology·Peng LiuYu-Guo Zheng

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