Metabolic engineering of Escherichia coli for the production of cinnamaldehyde.

Microbial Cell Factories
Hyun Bae BangKi Jun Jeong

Abstract

Plant parasitic nematodes are harmful to agricultural crops and plants, and may cause severe yield losses. Cinnamaldehyde, a volatile, yellow liquid commonly used as a flavoring or food additive, is increasingly becoming a popular natural nematicide because of its high nematicidal activity and, there is a high demand for the development of a biological platform to produce cinnamaldehyde. We engineered Escherichia coli as an eco-friendly biological platform for the production of cinnamaldehyde. In E. coli, cinnamaldehyde can be synthesized from intracellular L-phenylalanine, which requires the activities of three enzymes: phenylalanine-ammonia lyase (PAL), 4-coumarate:CoA ligase (4CL), and cinnamoyl-CoA reductase (CCR). For the efficient production of cinnamaldehyde in E. coli, we first examined the activities of enzymes from different sources and a gene expression system for the selected enzymes was constructed. Next, the metabolic pathway for L-phenylalanine biosynthesis was engineered to increase the intracellular pool of L-phenylalanine, which is a main precursor of cinnamaldehyde. Finally, we tried to produce cinnamaldehyde with the engineered E. coli. According to this result, cinnamaldehyde production as high as 75 mg/L c...Continue Reading

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Citations

May 14, 2016·Applied Microbiology and Biotechnology·Regina MahrJulia Frunzke
Feb 24, 2017·Journal of Agricultural and Food Chemistry·Wei ZhouYanhe Ma
Mar 30, 2017·Applied Microbiology and Biotechnology·Manuela GottardiEckhard Boles
Apr 5, 2017·Molecular Biotechnology·Neetu TyagiKrishna Jyoti Mukherjee
Apr 16, 2021·World Journal of Microbiology & Biotechnology·Liwen LiuPengfei Gu
May 7, 2021·Essays in Biochemistry·Dongsoo YangSang Yup Lee

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Methods Mentioned

BETA
deamination
affinity column chromatography
phosphotransferase
PCR
electrophoresis
gene knockout
X-ray

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