Metabolic engineering of Escherichia coli for the enhanced production of l-tyrosine

Biotechnology and Bioengineering
Byoungjin KimSang Yup Lee

Abstract

Despite wide applications of l-tyrosine in the market, microbial overproduction of l-tyrosine has been a great challenge due to the complex gene regulations involved in its biosynthetic pathway. To this end, effects of knocking out tyrR on the l-tyrosine production were further explored during the strain development. Also, blocking cellular uptake of l-tyrosine by knocking out tyrosine transporters was examined with respect to l-tyrosine production. Using feedback-resistant aroG and tyrA genes (aroGfbr and tyrAfbr hereafter) as initial overexpression targets, which encode 3-deoxy-7-phosphoheptulonate synthase and chorismate mutase or prephenate dehydrogenase, respectively, various combinations of genes were subsequently overexpressed in the Escherichia coli wild-type and tyrR knockout strain, and their effects on the l-tyrosine production were examined. Co-overexpression of aroGfbr , aroL and tyrC, a gene from Zymomonas mobilis functionally similar to tyrAfbr , but insensitive to l-tyrosine, led to the greatest l-tyrosine production regardless of the strains and plasmid constructs examined in this study. The strain BTY2.13 overexpressing the abovementioned three genes together with the removal of the l-tyrosine-specific transpo...Continue Reading

References

Nov 1, 1977·Journal of Bacteriology·M J Whipp, A J Pittard
Jan 1, 1977·Journal of Supramolecular Structure·T C Lo
Jan 1, 1992·Journal of Bacteriology·A Løbner-Olesen, M G Marinus
Aug 1, 1991·Journal of Bacteriology·A E AndrewsA J Pittard
Jan 1, 1986·Journal of Bacteriology·R C DeFeyter, J Pittard
Oct 1, 1970·Journal of Bacteriology·K D Brown
Feb 15, 1993·European Journal of Biochemistry·G ZhaoR A Jensen
Mar 1, 1996·Trends in Biotechnology·S Y Lee
May 1, 1997·Journal of Bacteriology·A J Cosgriff, A J Pittard
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Dec 21, 2004·Journal of Biotechnology·Hans Peter Sørensen, Kim Kusk Mortensen
Dec 23, 2004·Molecular Microbiology·James PittardJi Yang
Jan 16, 2007·Applied Microbiology and Biotechnology·Tina Lütke-Eversloh, Gregory Stephanopoulos
Oct 31, 2007·Applied Microbiology and Biotechnology·Tina Lütke-EverslohGregory Stephanopoulos
Dec 12, 2007·Biotechnology and Bioengineering·Ranjan PatnaikDavid F Kraynie
Apr 14, 2009·Nature Methods·Daniel G GibsonHamilton O Smith
Oct 12, 2010·Nature Methods·Daniel G GibsonChuck Merryman
Mar 29, 2011·Applied and Environmental Microbiology·Chin Giaw LimMattheos A G Koffas
Oct 25, 2011·Applied and Environmental Microbiology·Darmawi JuminagaJay D Keasling
May 19, 2012·Nature Chemical Biology·Jeong Wook LeeSang Yup Lee
Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Christine Nicole S SantosGregory Stephanopoulos
Jan 22, 2013·Nature Biotechnology·Dokyun NaSang Yup Lee
Oct 1, 2013·Nucleic Acids Research·Joseph P TorellaPamela A Silver
Sep 10, 2014·Microbial Cell Factories·Alberto RodriguezFrancisco Bolivar
Dec 21, 2014·Metabolic Engineering·Shujuan ZhaoMattheos A G Koffas
Jan 7, 2015·Current Opinion in Biotechnology·Rainer FischerStefan Schillberg
Oct 9, 2015·Nature Biotechnology·Sang Yup Lee, Hyun Uk Kim
Jun 1, 2017·Nature Communications·Zi Wei Luo, Sang Yup Lee

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Citations

Jul 13, 2019·Journal of Industrial Microbiology & Biotechnology·Yinbiao XuGuiyang Shi
Apr 17, 2020·Biotechnology Journal·Ying ZhuChun Li
Sep 21, 2018·Proceedings of the National Academy of Sciences of the United States of America·Dongsoo YangSang Yup Lee
Mar 15, 2020·Biotechnology Journal·Melanie MindtVolker F Wendisch
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Mar 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiaoqiang MaKang Zhou
Jan 29, 2020·Journal of Industrial Microbiology & Biotechnology·Xiaozhen LiuPengfei Gu
Jul 25, 2019·Nature Communications·Xiaoqiang MaKang Zhou
Nov 1, 2020·Applied Microbiology and Biotechnology·Xiaolei GuoJianzhong Huang
Apr 27, 2020·Metabolic Engineering·Christopher J RobinsonNigel S Scrutton
Apr 15, 2021·Microbial Cell Factories·Zimeng ZhangDawei Zhang
Jul 9, 2021·Journal of Agricultural and Food Chemistry·Fu-Xing NiuJian-Zhong Liu
Dec 21, 2021·Biotechnology and Bioengineering·Seon Young ParkSang Yup Lee

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