Metabolic engineering of Klebsiella pneumoniae for the de novo production of 2-butanol as a potential biofuel

Bioresource Technology
Zhen ChenDehua Liu

Abstract

Butanol isomers are important bulk chemicals and promising fuel substitutes. The inevitable toxicity of n-butanol and isobutanol to microbial cells hinders their final titers. In this study, we attempt to engineer Klebsiella pneumoniae for the de novo production of 2-butanol, another butanol isomer which shows lower toxicity than n-butanol and isobutanol. 2-Butanol synthesis was realized by the extension of the native meso-2,3-butanediol synthesis pathway with the introduction of diol dehydratase and secondary alcohol dehydrogenase. By the screening of different secondary alcohol dehydrogenases and diol dehydratases, 320mg/L of 2-butanol was produced by the best engineered K. pneumoniae. The production was increased to 720mg/L by knocking out the ldhA gene and appropriate addition of coenzyme B12. Further improvement of 2-butanol to 1030mg/L was achieved by protein engineering of diol dehydratase. This work lays the basis for the metabolic engineering of microorganism for the production of 2-butanol as potential biofuel.

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Citations

Jan 29, 2016·FEMS Microbiology Letters·George L Peabody, Katy C Kao
Aug 16, 2016·Current Opinion in Biotechnology·Zhen Chen, An-Ping Zeng
Jun 5, 2020·Biotechnology and Bioengineering·Xianghao WuMark A Eiteman
Jan 8, 2021·Biotechnology for Biofuels·Mobolaji Felicia AdegboyeOlubukola Oluranti Babalola
Nov 24, 2020·Frontiers in Bioengineering and Biotechnology·Abdul NasirTae Hyeon Yoo
Jun 3, 2021·Pathogens·Bruno Henrique Silva DiasChoong-Min Ryu
Feb 19, 2019·Industrial & Engineering Chemistry Research·Joana P C PereiraAdrie J J Straathof

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