Metabolic engineering of mannitol production in Lactococcus lactis: influence of overexpression of mannitol 1-phosphate dehydrogenase in different genetic backgrounds

Applied and Environmental Microbiology
H W WisselinkJ Hugenholtz

Abstract

To obtain a mannitol-producing Lactococcus lactis strain, the mannitol 1-phosphate dehydrogenase gene (mtlD) from Lactobacillus plantarum was overexpressed in a wild-type strain, a lactate dehydrogenase(LDH)-deficient strain, and a strain with reduced phosphofructokinase activity. High-performance liquid chromatography and (13)C nuclear magnetic resonance analysis revealed that small amounts (<1%) of mannitol were formed by growing cells of mtlD-overexpressing LDH-deficient and phosphofructokinase-reduced strains, whereas resting cells of the LDH-deficient transformant converted 25% of glucose into mannitol. Moreover, the formed mannitol was not reutilized upon glucose depletion. Of the metabolic-engineering strategies investigated in this work, mtlD-overexpressing LDH-deficient L. lactis seemed to be the most promising strain for mannitol production.

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Citations

Jul 7, 2009·Applied Microbiology and Biotechnology·Seung Hoon Song, Claire Vieille
Feb 25, 2010·Applied Microbiology and Biotechnology·Vicente MonederoMaría J Yebra
Nov 11, 2010·Applied Microbiology and Biotechnology·Badal C Saha, F Michael Racine
Apr 23, 2013·Applied Microbiology and Biotechnology·Maria Eugenia OrtizFernanda Mozzi
Jun 9, 2005·FEMS Microbiology Reviews·Ana Rute NevesHelena Santos
Dec 17, 2005·Nature Reviews. Microbiology·Bas Teusink, Eddy J Smid
Jan 31, 2007·Applied and Environmental Microbiology·Victor LaderoPascal Hols
Aug 16, 2011·Applied and Environmental Microbiology·Paula GasparHelena Santos
Mar 5, 2005·Applied and Environmental Microbiology·H Wouter WisselinkJeroen Hugenholtz
Nov 29, 2012·Bioresource Technology·Jing WangJong Moon Park
Sep 2, 2008·Current Opinion in Biotechnology·Olubolaji AkinterinwaPatrick Carmen Cirino

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