Metabolic engineering of oilseed crops to produce high levels of novel acetyl glyceride oils with reduced viscosity, freezing point and calorific value

Plant Biotechnology Journal
Jinjie LiuTimothy P Durrett

Abstract

Seed oils have proved recalcitrant to modification for the production of industrially useful lipids. Here, we demonstrate the successful metabolic engineering and subsequent field production of an oilseed crop with the highest accumulation of unusual oil achieved so far in transgenic plants. Previously, expression of the Euonymus alatus diacylglycerol acetyltransferase (EaDAcT) gene in wild-type Arabidopsis seeds resulted in the accumulation of 45 mol% of unusual 3-acetyl-1,2-diacyl-sn-glycerols (acetyl-TAGs) in the seed oil (Durrett et al., 2010 PNAS 107:9464). Expression of EaDAcT in dgat1 mutants compromised in their ability to synthesize regular triacylglycerols increased acetyl-TAGs to 65 mol%. Camelina and soybean transformed with the EaDAcT gene accumulate acetyl-triacylglycerols (acetyl-TAGs) at up to 70 mol% of seed oil. A similar strategy of coexpression of EaDAcT together with RNAi suppression of DGAT1 increased acetyl-TAG levels to up to 85 mol% in field-grown transgenic Camelina. Additionally, total moles of triacylglycerol (TAG) per seed increased 20%. Analysis of the acetyl-TAG fraction revealed a twofold reduction in very long chain fatty acids (VLCFA), consistent with their displacement from the sn-3 position b...Continue Reading

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Citations

Aug 12, 2015·Phytochemistry·Mike PollardYair Shachar-Hill
Apr 9, 2016·International Journal of Molecular Sciences·Grace Q ChenJiann-Tsyh Lin
Oct 30, 2016·Biochimie·Eberhard MunzLjudmilla Borisjuk
Sep 9, 2016·Annual Review of Microbiology·Gina R LewinCameron R Currie
Aug 16, 2016·The Plant Journal : for Cell and Molecular Biology·Sateesh KagaleIsobel A P Parkin
Apr 27, 2017·Plant & Cell Physiology·J A Aznar-Moreno, T P Durrett
Feb 13, 2018·Plant Reproduction·Sébastien Baud
Sep 20, 2018·Plant Biotechnology Journal·Michaela McGinnJohn C Sedbrook
Jul 18, 2017·The Plant Journal : for Cell and Molecular Biology·Tam N T TranTimothy P Durrett
Sep 17, 2016·Science·Patrick J Horn, Christoph Benning
Mar 24, 2017·Biotechnology for Biofuels·Tam N T TranTimothy P Durrett
Aug 3, 2016·The Plant Journal : for Cell and Molecular Biology·Richard P HaslamJohnathan A Napier
Dec 14, 2018·BMC Plant Biology·Monica A Schmidt, Eliot M Herman
May 12, 2019·Plant & Cell Physiology·Xue-Rong ZhouSurinder Singh
Aug 19, 2017·Plant Science : an International Journal of Experimental Plant Biology·Jose A Aznar-Moreno, Timothy P Durrett
Jul 9, 2020·Progress in Lipid Research·Sandra M CorreaYariv Brotman
Feb 24, 2021·The Plant Journal : for Cell and Molecular Biology·Linah AlkotamiTimothy P Durrett

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