Metabolic engineering of Phaeodactylum tricornutum for the enhanced accumulation of omega-3 long chain polyunsaturated fatty acids

Metabolic Engineering
Mary L HamiltonOlga Sayanova

Abstract

We have engineered the diatom Phaeodactylum tricornutum to accumulate the high value omega-3 long chain polyunsaturated fatty acid docosahexaenoic acid (DHA). This was achieved by the generation of transgenic strains in which the Δ5-elongase from the picoalga Ostreococcus tauri was expressed to augment the endogenous fatty acid biosynthetic pathway. Expression of the heterologous elongase resulted in an eight-fold increase in docosahexaenoic acid content, representing a marked and valuable change in the fatty acid profile of this microalga. Importantly, DHA was shown to accumulate in triacylglycerols, with several novel triacylglycerol species being detected in the transgenic strains. In a second iteration, co-expression of an acyl-CoA-dependent Δ6-desaturase with the Δ5-elongase further increased DHA levels. Together, this demonstrates for the first time the potential of using iterative metabolic engineering to optimise omega-3 content in algae.

References

Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·T R Larson, I A Graham
May 3, 2007·Annual Review of Plant Biology·Johnathan A Napier
Dec 10, 2008·Biochimie·John L Harwood, Irina A Guschina
Aug 18, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Serge M Garcia, Andrew A Rosenberg
Oct 26, 2010·Metabolic Engineering·Randor RadakovitsMatthew C Posewitz
Feb 16, 2012·The Plant Journal : for Cell and Molecular Biology·Michele FabrisGino J E Baart

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Citations

Jun 3, 2014·The Proceedings of the Nutrition Society·Jacques Delarue, Nathalie Guriec
Aug 30, 2014·Trends in Biotechnology·A J KlokR H Wijffels
Mar 19, 2015·Marine Drugs·Lina-Juana Dolch, Eric Maréchal
Aug 8, 2015·Biotechnology Advances·Hu-Hu LiuHe Huang
Sep 1, 2015·Current Opinion in Biotechnology·Jennifer LeveringKarsten Zengler
Feb 24, 2016·European Journal of Lipid Science and Technology : EJLST·Johnathan A NapierOlga Sayanova
Mar 14, 2016·Environment International·Jillian P FryRobert S Lawrence
Apr 12, 2016·Frontiers in Microbiology·Chiranjib BanerjeePratyoosh Shukla
Feb 3, 2015·The Plant Journal : for Cell and Molecular Biology·Mark A ScaifeAlison G Smith
Dec 3, 2014·Biotechnology Advances·Stamatia BellouGeorge Aggelis
Aug 28, 2014·Journal of Biotechnology·Lutz Wobbe, Claire Remacle
Aug 12, 2014·Progress in Lipid Research·Yangmin GongFenghong Huang
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Miriam Payá-MilansMónica Venegas-Calerón
Oct 6, 2016·Critical Reviews in Biotechnology·Ellis C O'Neill, Steven Kelly
Nov 9, 2016·Applied Microbiology and Biotechnology·Janeth I GalarzaVitalia Henríquez
Dec 17, 2016·Marine Drugs·Weiqi FuKourosh Salehi-Ashtiani
Feb 28, 2017·Journal of Oleo Science·Yu-Hong YangHiroyuki Taroda
Jan 25, 2018·Marine Drugs·Li-Juan WangFu-Li Li
Apr 21, 2018·Critical Reviews in Food Science and Nutrition·Ming-Hua LiangJian-Guo Jiang
Nov 2, 2014·Molecular BioSystems·Joris BeldMichael D Burkart
Nov 23, 2017·Biotechnology for Biofuels·Seungjib JeonYong Keun Chang
May 20, 2015·Journal of Biological Research·Sergey M ShishlyannikovAlexander G Gorshkov
Feb 10, 2018·Applied Microbiology and Biotechnology·Marianna DourouGeorge Aggelis
May 10, 2019·Marine Drugs·Chiara LauritanoAlessandra Rogato
May 30, 2019·Molecular Biotechnology·Vikas Kumar PatelBhaskar Bhadra
Jul 19, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Weichao Huang, Fayza Daboussi
Nov 29, 2015·Biochemical Society Transactions·Dipali SinghMark Poolman

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Methods Mentioned

BETA
transgenic
acylation

Software Mentioned

Agilent ChemStation

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