Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds

Biotechnology for Biofuels
Sandra Isabel González-MoralesDamar López-Arredondo

Abstract

The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by public and private sectors to develop this field. However, it has been a challenge to develop a viable and cost-effective platform for cultivation of cyanobacteria and microalgae under outdoor conditions. Dealing with contamination caused by bacteria, weedy algae/cyanobacteria and other organisms is a major constraint to establish effective cultivation processes. Here, we describe the implementation in the cyanobacterium Synechococcus elongatus PCC 7942 of a phosphorus selective nutrition system to control biological contamination during cultivation. The system is based on metabolic engineering of S. elongatus to metabolize phosphite, a phosphorus source not normally metabolized by most organisms, by expressing a bacterial phosphite oxidoreductase (PtxD). Engineered S. elongatus strains expressing PtxD grow at a similar rate on media supplemented with phosphite as the non-transformed control supplemented with phosphate. We show that when grown in media containing phosphite as the sole phosphorus source in glass flasks, the engi...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 1, 1971·Bacteriological Reviews·R Y StanierG Cohen-Bazire
Jan 5, 2002·Canadian Journal of Microbiology·A E McDonaldW C Plaxton
Jul 13, 2002·Plant Physiology·Deepa K VaradarajanKashchandra G Raghothama
Aug 20, 2004·Journal of Bacteriology·Andrea K White, William W Metcalf
Feb 6, 2007·Applied Microbiology and Biotechnology·José A Del CampoMiguel G Guerrero
Apr 10, 2007·Methods in Molecular Biology·Eugenia M ClericoSusan S Golden
Nov 20, 2009·Environmental Science & Technology·Herbe PechKrishna L Foster
Aug 24, 2010·Biotechnology Advances·Niels-Henrik NorskerRené H Wijffels
Jan 8, 2011·Trends in Biotechnology·Daniel C DucatPamela A Silver
Oct 19, 2011·Environmental Microbiology·Asunción MartínezSallie W Chisholm
Jan 1, 2011·Frontiers in Plant Science·Laurent NussaumeMarie-Christine Thibaud
Aug 28, 2012·Nature Biotechnology·Damar Lizbeth López-Arredondo, Luis Herrera-Estrella
Nov 29, 2012·Bioresource Technology·Hui WangTianzhong Liu
Dec 25, 2012·Bioresource Technology·C V González-LópezE Molina-Grima
May 8, 2013·Environmental Science & Technology·Chao HanXiaorong Wang
May 21, 2013·Journal of Bacteriology·Maria R Gomez-GarciaDevaki Bhaya
Feb 7, 2014·Biotechnology for Biofuels·Leonardo Brantes Bacellar Mendes, Alane Beatriz Vermelho
Jun 14, 2014·Bioresource Technology·Nathan G SchoeppMichael D Burkart
Oct 15, 2014·Proceedings of the National Academy of Sciences of the United States of America·Matthew A PasekZachary Atlas
Jan 13, 2015·Plant Science : an International Journal of Experimental Plant Biology·Maribel M Loera-QuezadaLuis Herrera-Estrella
Jun 18, 2015·Environmental Microbiology Reports·Despo PolyviouThomas S Bibby
Mar 24, 2016·Plant Biotechnology Journal·Maribel M Loera-QuezadaLuis Herrera-Estrella
Feb 13, 2017·Advances in Applied Microbiology·I A Figueroa, J D Coates
Nov 25, 2017·Nature Communications·Claudine BissonAndrew Hitchcock
Jan 8, 2018·Bioresource Technology·Tan Phat LamJo-Shu Chang
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Devendra PandeyaKeerti S Rathore

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Methods Mentioned

BETA
chemical treatments
transgenics
transgenic
PCR
Fluorescence
light microscopy
scraping
electrophoresis

Software Mentioned

SEMARNAT
MIC qPCR Cycler
- Codon optimization Tool
BIOLINE
Basic Local Alignment Search Tool ( BLASTN )
[UNK]
Genious
BLAST
Primer

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