Metabolic engineering of proanthocyanidin production by repressing the isoflavone pathways and redirecting anthocyanidin precursor flux in legume

Plant Biotechnology Journal
Penghui LiJian Zhao

Abstract

MtPAR is a proanthocyanidin (PA) biosynthesis regulator; the mechanism underlying its promotion of PA biosynthesis is not fully understood. Here, we showed that MtPAR promotes PA production by a direct repression of biosynthesis of isoflavones, the major flavonoids in legume, and by redirecting immediate precursors, such as anthocyanidins, flux into PA pathway. Ectopic expression of MtPAR repressed isoflavonoid production by directly binding and suppressing isoflavone biosynthetic genes such as isoflavone synthase (IFS). Meanwhile, MtPAR up-regulated PA-specific genes and decreased the anthocyanin levels without altering the expression of anthocyanin biosynthetic genes. MtPAR may shift the anthocyanidin precursor flux from anthocyanin pathway to PA biosynthesis. MtPAR complemented PA-deficient phenotype of Arabidopsis tt2 mutant seeds, demonstrating their similar action on PA production. We showed the direct interactions between MtPAR, MtTT8 and MtWD40-1 proteins from Medicago truncatula and Glycine max, to form a ternary complex to trans-activate PA-specific ANR gene. Finally, MtPAR expression in alfalfa (Medicago sativa) hairy roots and whole plants only promoted the production of small amount of PAs, which was significantly ...Continue Reading

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Citations

Apr 21, 2017·Analytical and Bioanalytical Chemistry·Gaoyang ZhangJian Zhao
Nov 21, 2017·Journal of Dairy Science·Charles G Schwab, Glen A Broderick
Nov 21, 2017·Animal : an International Journal of Animal Bioscience·G A Broderick
Jul 26, 2018·International Journal of Molecular Sciences·Marwa A IsmaelCheng Xiao Hu
Dec 17, 2020·Journal of Integrative Plant Biology·Nai-Qian Dong, Hong-Xuan Lin
Aug 14, 2019·Journal of Agricultural and Food Chemistry·Chao ZhengLiang Chen

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Methods Mentioned

BETA
glycosylation
transgenic
one‐hybrid
Y2H
PCR
Chip

Software Mentioned

MET
MtPAR
SDS
IDEA

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