Metabolic engineering of proanthocyanidins through co-expression of anthocyanidin reductase and the PAP1 MYB transcription factor

The Plant Journal : for Cell and Molecular Biology
De-Yu XieRichard A Dixon

Abstract

Proanthocyanidins (PAs) and their monomeric building blocks, the (epi)-flavan-3-ols, are plant antioxidants that confer multiple human health benefits. The presence of PAs in forage crops is an important agronomic trait, preventing pasture bloat in ruminant animals. However, many consumed plant materials lack PAs, and there has been little success to date in introducing monomeric or polymeric flavan-3-ols de novo into plant tissues for disease prevention by dietary means or development of 'bloat-safe' forages. We report the introduction of PAs into plants by combined expression of a MYB family transcription factor and anthocyanidin reductase for conversion of anthocyanidin into (epi)-flavan-3-ol. Tobacco leaves expressing both transgenes accumulated epicatechin and gallocatechin monomers, and a series of dimers and oligomers consisting primarily of epicatechin units. The levels of PAs reached values that would confer bloat reduction in forage species. Expression of anthocyanidin reductase in anthocyanin-containing leaves of the forage legume Medicago truncatula resulted in production of a specific subset of PA oligomers.

References

Mar 1, 1988·Tubercle·J M Grange
Jul 29, 1998·Biochemistry and Molecular Biology International·E CossinsL Packer
May 8, 1999·Free Radical Biology & Medicine·M BagchiS J Stohs
Jun 22, 2000·Skin Pharmacology and Applied Skin Physiology·T TakahashiY Yokoo
Jun 28, 2003·Natural Product Reports·Karin SpringobKazuki Saito
Aug 29, 2003·Phytochemistry·M A Susan MarlesMargaret Y Gruber
Aug 29, 2003·Nature·Mauro SerafiniAlan Crozier
Dec 17, 2003·The Plant Journal : for Cell and Molecular Biology·Satoshi KitamuraAtsushi Tanaka
Jan 17, 2004·Archives of Biochemistry and Biophysics·De-Yu XieRichard A Dixon
Feb 24, 2004·Journal of Medicinal Food·John ShiYukio Kakuda
Mar 9, 2004·Current Opinion in Plant Biology·Pierre Broun
Dec 4, 2004·Planta·Naveed AzizRichard A Dixon
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ivan R BaxterJeffrey F Harper
Feb 22, 2005·The New Phytologist·Richard A DixonShashi B Sharma
Mar 17, 2005·Journal of Agricultural and Food Chemistry·Elvira Gonzalez de MejiaHideka Kobayashi
Apr 19, 2005·Biotechnology Letters·Takayuki TohgeKazuki Saito
Sep 13, 2005·Phytochemistry·De-Yu Xie, Richard A Dixon
Sep 20, 2005·The Plant Journal : for Cell and Molecular Biology·Shashi B Sharma, Richard A Dixon

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Citations

Mar 24, 2010·Applied Microbiology and Biotechnology·Hai DuYixiong Tang
Nov 28, 2012·Functional & Integrative Genomics·Lei ZhaoTao Xia
Feb 1, 2011·Journal of Applied Genetics·Suresh Kumar
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jerome VerdierMichael K Udvardi
Sep 22, 2007·Plant Physiology·Yongzhen PangRichard A Dixon
Feb 13, 2009·Plant Physiology·Felix LippoldMichael K Udvardi
Jun 16, 2009·Plant Physiology·Philippe HugueneyAgnès Ageorges
Dec 15, 2011·Plant Physiology·Shamila Weerakoon AbeynayakeGerman Spangenberg
Oct 28, 2006·Annual Review of Plant Biology·Diter von Wettstein
Dec 1, 2011·Bioengineered Bugs·De-Yu Xie, Ming-Zhu Shi
Jan 31, 2012·Plant Science : an International Journal of Experimental Plant Biology·Erin EgelkroutJohn A Howard
Dec 3, 2014·Metabolic Engineering·Ling Yuan, Erich Grotewold
May 8, 2007·Biochemical and Biophysical Research Communications·Yue-Hua XiaoYan Pei
Mar 27, 2007·Mutation Research·Kevin M Davies
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Oliver Yu, Joseph M Jez
Oct 1, 2008·The New Phytologist·Ping XuMarilyn J Roossinck
Mar 19, 2008·Plant Biotechnology Journal·Michal Moyal Ben ZviAlexander Vainstein
Dec 22, 2006·The Plant Journal : for Cell and Molecular Biology·Richard V EspleyAndrew C Allan
Dec 17, 2011·Journal of the Science of Food and Agriculture·XianLin ZhangTao Xia
Jan 1, 2014·Plant Biotechnology Journal·Wusheng LiuCharles Neal Stewart
Apr 1, 2008·Current Opinion in Biotechnology·Shuiqin Wu, Joe Chappell
Aug 1, 2006·Current Opinion in Plant Biology·Li-Jia Qu, Yu-Xian Zhu
Apr 1, 2008·Current Opinion in Biotechnology·Erich Grotewold
Apr 12, 2014·Plant Signaling & Behavior·Wenjia XuChristian Dubos
Mar 26, 2015·Applied Microbiology and Biotechnology·Meiliang ZhouYanmin Wu
Nov 27, 2014·Frontiers in Plant Science·Aidyn Mouradov, German Spangenberg

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