Metabolic engineering of Rhodopseudomonas palustris for squalene production

Journal of Industrial Microbiology & Biotechnology
Wen XuYang Wang

Abstract

Squalene is a strong antioxidant used extensively in the food, cosmetic and medicine industries. Rhodopseudomonas palustris TIE-1 was used as the host because of its ability to grow photosynthetically using solar energy and carbon dioxide from atmosphere. The deletion of the shc gene resulted in a squalene production of 3.8 mg/g DCW, which was 27-times higher than that in the wild type strain. For constructing a substrate channel to elevate the conversion efficiency, we tried to fuse crtE gene with hpnD gene. By fusing the two genes, squalene content was increased to 12.6 mg/g DCW, which was 27.4 % higher than that resulted from the co-expression method. At last, the titer of squalene reached 15.8 mg/g DCW by co-expressing the dxs gene, corresponding to 112-fold increase relative to that for wild-type strain. This study provided novel strategies for improving squalene yield and demonstrated the potential of producing squalene by Rhodopseudomonas palustris.

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Citations

Oct 13, 2016·World Journal of Microbiology & Biotechnology·Wen XuYang Wang
Aug 2, 2019·International Journal of Cosmetic Science·F MellouS Papageorgiou
Apr 11, 2019·Frontiers in Bioengineering and Biotechnology·Nisarg GohilVijai Singh
Jan 26, 2021·Frontiers in Bioengineering and Biotechnology·Sheida StephensD Grant Allen
Jun 15, 2021·Applied Microbiology and Biotechnology·Peng-Wei HuangHe Huang

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