PMID: 9164848May 15, 1997Paper

Metabolic evidence for PtdIns(4,5)P2-directed phospholipase C in permeabilized plant protoplasts

The Biochemical Journal
C A BrearleyD E Hanke

Abstract

Comparison of the sequences of the genes encoding phospholipase C (PLC) which have been cloned to date in plants with their mammalian counterparts suggests that plant PLC is similar to PLCdelta of mammalian cells. The physiological role and mechanism of activation of PLCdelta is unclear. It has recently been shown that Ins(1,4,5)P3 may not solely be the product of PtdIns(4,5)P2-directed PLC activity. Enzyme activities capable of producing Ins(1,4,5)P3 from endogenous inositol phosphates are present in Dictyostelium and also in rat liver. Significantly it has not been directly determined whether Ins(1,4,5)P3 present in higher plants is the product of a PtdIns(4, 5)P2-directed PLC activity. Therefore we have developed an experimental strategy for the identification of d-Ins(1,4,5)P3 in higher plants. By the use of a short-term non-equilibrium labelling strategy in permeabilized plant protoplasts, coupled to the use of a 'metabolic trap' to prevent degradation of [32P]Ins(1,4,5)P3, we were able to determine the distribution of 32P in individual phosphate esters of Ins(1,4,5)P3. The [32]Ins(1,4,5)P3 identified showed the same distribution of label in individual phosphate esters as that of [32P]PtdIns(4,5)P2 isolated from the same t...Continue Reading

Citations

May 5, 2000·Molecular Plant-microbe Interactions : MPMI·T Shigaki, M K Bhattacharyya
May 21, 2018·The Plant Journal : for Cell and Molecular Biology·Hui-Fen KuoTzyy-Jen Chiou
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·F Lemtiri-ChliehC A Brearley
Mar 10, 2020·Food Science & Nutrition·Liang ShuaiXuemei He
Jan 1, 2002·The Arabidopsis Book·Liming Xiong, Jian-Kang Zhu
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·I StaxenM R McAinsh
Jul 1, 2001·The New Phytologist·Jason J Rudd, Vernonica E Franklin-Tong
Jun 6, 2000·Trends in Plant Science·J M StevensonW F Boss
Mar 6, 1999·Current Opinion in Plant Biology·A J Trewavas, R Malhó

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