Metabolic fate of the new synthetic cannabinoid 7'N-5F-ADB in rat, human, and pooled human S9 studied by means of hyphenated high-resolution mass spectrometry

Drug Testing and Analysis
Lilian H J RichterMarkus R Meyer

Abstract

New psychoactive substances (NPS) are an important issue in clinical/forensic toxicology. 7'N-5F-ADB, a synthetic cannabinoid derived from 5F-ADB, appeared recently on the market. Up to now, no data about its mass spectral fragmentation pattern, metabolism, and thus suitable targets for toxicological urine screenings have been available. Therefore, the aim of this study was to elucidate the metabolic fate of 7'N-5F-ADB in rat, human, and pooled human S9 (pS9). The main human urinary excretion products, which can be used as targets for toxicological screening procedures, were identified by Orbitrap (OT)-based liquid chromatography-high resolution-tandem mass spectrometry (LC-HRMS/MS). In addition, possible differentiation of 7'N-5F-ADB and 5F-ADB via LC-HRMS/MS was studied. Using the in vivo and in vitro models for metabolism studies, 36 metabolites were tentatively identified. 7'N-5F-ABD was extensively metabolized in rat and human with minor species differences observed. The unchanged parent compound could be found in human urine but metabolites were far more abundant. The most abundant ones were the hydrolyzed ester (M5), the hydrolyzed ester in combination with hydroxylation of the tertiary butyl part (M11), and the hydrolyz...Continue Reading

References

May 3, 2011·Analytical and Bioanalytical Chemistry·Dirk K WissenbachHans H Maurer
Aug 11, 2015·Forensic Toxicology·Koutaro HasegawaOsamu Suzuki
Feb 14, 2017·Forensic Science International : Synergy·Bernardino BarcelóManuela Pellegrini

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Citations

Jul 21, 2020·Journal of Analytical Toxicology·Aitor Sorribes-SorianoFrancesc A Esteve-Turrillas
Oct 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu Mi ParkJennifer Herrmann
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Apr 2, 2021·Analytical and Bioanalytical Chemistry·Lea WagmannMarkus R Meyer
Nov 7, 2020·Journal of Mass Spectrometry : JMS·David Fabregat-SafontMaría Ibáñez

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