Metabolic fingerprint of insulin resistance in human polymorphonuclear leucocytes

PloS One
Martina Palomino-SchätzleinJosé Raúl Herance

Abstract

The present study was aimed at determining the metabolic profile of PMNs in obese subjects, and to explore its potential relationship with insulin resistance (IR). To achieve this goal, a pilot clinical study was performed using PMNs from 17 patients with obesity and IR, and 17 lean controls without IR, which was validated in an additional smaller cohort (consisting of 10 patients and 10 controls). PMNs were isolated from peripheral blood and nuclear magnetic resonance was used to perform the metabolomic analysis. A total of 48 metabolites were quantified. The main metabolic change found in PMNs was a significant increase in 2-aminoisobutyric acid with a direct correlation with HOMA-IR (p<0.001), BMI (p<0.000001) and waist circumference (p<0.000001). By contrast, a decrease of 3-hydroxyisovalerate was observed with an inverse correlation with HOMA-IR (p = 0.001), BMI (p = 0.001) and waist circumference (p = 0.0001). Notably, the metabolic profile in plasma was different than that obtained in PMNs. In summary, our results suggest that the change in 3-hydroxyisovalerate and 2-aminoisobutyric is the key metabolic fingerprint in PMNs of obese subjects with IR. In addition, our methodology could be an easy and reliable tool for moni...Continue Reading

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Citations

Oct 11, 2019·Biomarkers in Medicine·Fabrizio MontecuccoFederico Carbone
May 31, 2020·Journal of Clinical Medicine·Martina Palomino-SchätzleinJosé Raúl Herance
Nov 12, 2019·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Martina Palomino-SchätzleinJosé-Manuel Fernández-Real

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
PCA
flow cytometry

Software Mentioned

Topspin3
SIMCA
MetPA
MestreNova
Metaboanalyst
R
PLS
R commander
DA

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