Metabolic flux analysis and metabolic engineering of microorganisms

Molecular BioSystems
Hyun Uk KimSang Yup Lee

Abstract

Recent advances in metabolic flux analysis including genome-scale constraints-based flux analysis and its applications in metabolic engineering are reviewed. Various computational aspects of constraints-based flux analysis including genome-scale stoichiometric models, additional constraints used for the improved accuracy, and several algorithms for identifying the target genes to be manipulated are described. Also, some of the successful applications of metabolic flux analysis in metabolic engineering are reviewed. Finally, we discuss the limitations that need to be overcome to make the results of genome-scale flux analysis more realistically represent the real cell metabolism.

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Citations

Mar 15, 2008·Applied Microbiology and Biotechnology·Sang Yup LeeYu-Sin Jang
Sep 8, 2010·Applied Microbiology and Biotechnology·Michael KohlstedtChristoph Wittmann
Nov 30, 2011·Applied Microbiology and Biotechnology·Brett A BoghigianBlaine A Pfeifer
Nov 29, 2012·Applied Microbiology and Biotechnology·Chuan XuMing Chen
Aug 26, 2010·Molecular Biotechnology·Chen-Hsiang Yeang
Oct 9, 2013·Journal of Theoretical Biology·Kristo AbnerRaivo Vilu
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jong Myoung ParkSang Yup Lee
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Jun 19, 2012·BMC Systems Biology·Amornpan KlanchuiAsawin Meechai
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May 26, 2009·Biotechnology Advances·Jong Myoung ParkSang Yup Lee
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