Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons

Plant, Cell & Environment
Vidya V IyerJacqueline V Shanks

Abstract

Metabolic flux maps developed from 13C metabolic flux analysis (13C MFA) are effective tools for assessing the response of biological systems to genetic or environmental perturbations, and for identifying possible metabolic engineering targets. Experimental treatments were designed to distinguish between temperature effects prior to, and during incubation in vitro, on primary metabolism in developing soybeans. Biomass accumulation increased with temperature as did carbon partitioning into lipids. The flux through the plastidic oxidative pentose phosphate pathway (pgl(P)) relative to sucrose intake remained fairly constant [ approximately 56% (+/-24%)] when cotyledons were transferred from an optimum growth temperature to varying temperatures in in vitro culture, signifying a rigid node under these conditions. However, pgl(P) flux ranged from 57 to 77% of sucrose intake when growth temperature in planta varied and were cultured in vitro at the same temperature (as the plant), indicating a flexible node for this case. The carbon flux through the anaplerotic reactions catalysed by plastidic malic enzyme (me(P)), cytosolic phosphoenolpyruvate (PEP) carboxylase and the malate (Mal) transporter from the cytosol to mitochondrion varie...Continue Reading

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Mar 1, 2012·Journal of Experimental Botany·John O'GradyJohn A Morgan
Jun 7, 2013·Journal of Experimental Botany·Quyen TruongJacqueline V Shanks
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