Metabolic pathway of xenoestrogenic short ethoxy chain-nonylphenol to nonylphenol by aerobic bacteria, Ensifer sp. strain AS08 and Pseudomonas sp. strain AS90

Applied Microbiology and Biotechnology
Xin LiuFusako Kawai

Abstract

Ensifer sp. strain AS08 and Pseudomonas sp. strain AS90 degrading short ethoxy (EO) chain-nonylphenol (NP) [NPEO(av2.0) containing NP mono- approximately tetraethoxylates (NP1EO approximately NP4EO); average 2.0 EO units] were isolated by enrichment cultures. Both strains grew on NP but not on octyl- and nonylphenol polyethoxylates (NPEOs) (average 10 EO units). Growth and degradation of NPEO(av2.0) was increased with increased concentrations of yeast extract (0.02-0.5%) in a culture medium. Culture supernatants of both strains grown on NPEO(av2.0) were analyzed by high-performance liquid chromatography, showing degradation of NP4EO-NP1EO. The metabolites from nonylphenol diethoxylate (NP2EO) by resting cells of both strains were identified by gas chromatography-mass spectrometry as nonylphenoxyethoxyacetic acid, NP1EO, nonylphenoxyacetic acid (NP1EC), and NP, while those from NP1EO were identified as NP1EC and NP. Cell-free extracts from strain AS08 grown on NPEO(av2.0) dehydrogenated NPEOs, NPEO(av2.0), NP2EO, NP1EO, and PEG 400, but the extracts were inactive toward di- approximately tetraethylene glycol. Aldehydes were formed in the reaction mixture of each substrate with cell-free extracts. From these results, the aerobic ...Continue Reading

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Citations

Jun 26, 2008·Journal of Environmental Sciences (China)·Yi LiuJianfu Zhao
Aug 8, 2013·Archives of Environmental Contamination and Toxicology·Adeline ColinXavier Dauchy
Aug 15, 2013·Environmental Science and Pollution Research International·Yu ZhangMin Yang
Jul 6, 2010·Biochemical Pharmacology·Emily PowellWei Xu
Mar 11, 2016·Environmental Science and Pollution Research International·Naling BaiYuhua Zhao
Jun 9, 2009·Environmental Pollution·Vesna Micić, Thilo Hofmann
Oct 27, 2009·Marine Pollution Bulletin·Seongjin HongKyung-Hoon Shin

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