Metabolic profiles and phylogenetic diversity of microbial communities from chlorinated pesticides contaminated sites of different geographical habitats of India

Journal of Applied Microbiology
N ManickamR Shanker

Abstract

To study the microbial communities in three sites contaminated with chlorinated pesticides and evaluation of dehydrodechlorinase (linA) gene variants involved in gamma-hexachlorocyclohexane (γ-HCH, lindane) degradation. Using a culture-independent method, 16S rRNA genes were amplified from microbial communities occurring in contaminated soils. From 375 clone libraries analysed, 55 different restriction fragment length polymorphism phylotypes were obtained. Dehydrodechlorinase (linA) gene, which initiates the γ-HCH degradation, was directly amplified by PCR from the DNA extracted from soils. Deduced amino acid sequences of eight variant genotypes of linA showed few amino acid changes. All the variants of linA had mutations of F151L and S154T, and one of the genotype carried 12 amino acid changes when compared to a linA of Sphingomonas sp. reported from the same soil. The microbial communities displayed complex and diverse groups similar to bacteria involved in biodegradation. The presence of biodegradative genes like linA indicates the presence of communities with capacity to biodegrade the persistent pesticide HCH. This study provides insights to evaluate the presence of catabolic genes and assessing the bioremediation potentia...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Apr 1, 1991·Clinics in Dermatology·R P Rapini
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 17, 1999·Chemosphere·S P KaleF P Carvalho
Dec 1, 2000·Applied and Environmental Microbiology·E A GreeneG Voordouw
Jun 26, 2001·Journal of Industrial Microbiology & Biotechnology·Y NagataM Takagi
Aug 14, 2002·Journal of Applied Microbiology·T D SutherlandJ G Oakeshott
Nov 30, 2005·Applied Microbiology and Biotechnology·N ManickamM Schlömann
Dec 14, 2005·Environmental Microbiology·William W MohnVictor de Lorenzo
Jul 20, 2007·Applied Microbiology and Biotechnology·Yuji NagataMasataka Tsuda
Nov 29, 2008·Nurse Education in Practice·Karen Holland
Jul 1, 1985·Evolution; International Journal of Organic Evolution·Joseph Felsenstein

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Citations

Aug 13, 2011·Antonie van Leeuwenhoek·Natesan ManickamShanmugam Mayilraj
May 9, 2014·Archives of Microbiology·Natesan ManickamShanmugam Mayilraj
May 28, 2014·Current Opinion in Biotechnology·Carsten Suhr Jacobsen, Mathis Hjort Hjelmsø
Jul 27, 2017·Journal of Basic Microbiology·Bo XuZunxi Huang
Jan 19, 2018·Current Microbiology·Mara R de Lima E SilvaMaria B A Varesche
Oct 12, 2016·Microbiology Spectrum·Tasha M Santiago-Rodriguez, Raúl J Cano

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