Metabolic Profiling and Enzyme Analyses Indicate a Potential Role of Antioxidant Systems in Complementing Glyphosate Resistance in an Amaranthus palmeri Biotype

Journal of Agricultural and Food Chemistry
Amith S MaroliNishanth Tharayil

Abstract

Metabolomics and biochemical assays were employed to identify physiological perturbations induced by a commercial formulation of glyphosate in susceptible (S) and resistant (R) biotypes of Amaranthus palmeri. At 8 h after treatment (HAT), compared to the respective water-treated control, cellular metabolism of both biotypes were similarly perturbed by glyphosate, resulting in abundance of most metabolites including shikimic acid, amino acids, organic acids and sugars. However, by 80 HAT the metabolite pool of glyphosate-treated R-biotype was similar to that of the control S- and R-biotypes, indicating a potential physiological recovery. Furthermore, the glyphosate-treated R-biotype had lower reactive oxygen species (ROS) damage, higher ROS scavenging activity, and higher levels of potential antioxidant compounds derived from the phenylpropanoid pathway. Thus, metabolomics, in conjunction with biochemical assays, indicate that glyphosate-induced metabolic perturbations are not limited to the shikimate pathway, and the oxidant quenching efficiency could potentially complement the glyphosate resistance in this R-biotype.

References

Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J GörlachJ Schmid
Jan 23, 2003·Journal of Agricultural and Food Chemistry·Thomas C MuellerC Neal Stewart
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·William C. Plaxton
Jan 22, 2005·Pest Management Science·Gerald M Dill
Dec 6, 2005·Plant Physiology and Biochemistry : PPB·Nuria de MaríaMercedes Fernández-Pascual
Apr 5, 2007·Nature Protocols·Jan LisecAlisdair R Fernie
Feb 14, 2008·The Plant Journal : for Cell and Molecular Biology·Oliver FiehnBasil Nikolau
Feb 15, 2008·Pest Management Science·Stephen O Duke, Stephen B Powles
Jul 19, 2008·Journal of Agricultural and Food Chemistry·Jin ZhuPatrick J Tranel
Aug 30, 2008·Plant Physiology and Biochemistry : PPB·Nagib AhsanByung-Hyun Lee
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Todd A GainesPhilip Westra
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Sep 29, 2011·Journal of Plant Physiology·Luis OrcarayMercedes Royuela
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·Gary J PattiGary Siuzdak
May 4, 2012·Nucleic Acids Research·Jianguo XiaDavid S Wishart
May 18, 2012·Metabolomics : Official Journal of the Metabolomic Society·Ewa SzymańskaJohan A Westerhuis
Jul 24, 2012·Plant Physiology·Laura A LallemandAndrew A McCarthy
Jul 9, 2013·Trends in Genetics : TIG·Christophe DélyeValérie Le Corre

❮ Previous
Next ❯

Citations

Dec 15, 2015·Journal of Agricultural and Food Chemistry·Manuel Fernández-EscaladaMercedes Royuela
Mar 7, 2017·Frontiers in Plant Science·Marcelo P GomesPhilippe Juneau
Feb 10, 2019·The New Phytologist·Martin M Vila-AiubStephen B Powles
Dec 19, 2019·Plants·Eric L PattersonTodd A Gaines
Mar 6, 2019·International Journal of Molecular Sciences·Andréia CaverzanLeandro Vargas
Mar 17, 2019·Metabolomics : Official Journal of the Metabolomic Society·Siriwat BoonchaisriDaniel A Dias
May 8, 2021·Frontiers in Plant Science·Ashwini Sushil Narvekar, Nishanth Tharayil
Sep 16, 2017·Pesticide Biochemistry and Physiology·Ana ZabalzaMercedes Royuela
Aug 10, 2021·NMR in Biomedicine·Amith Sadananda Maroli, Robert Powers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Analytical and Bioanalytical Chemistry
Rainer SchuhmacherRoyston Goodacre
© 2021 Meta ULC. All rights reserved