Metabolic Profiling in Formalin-Fixed and Paraffin-Embedded Prostate Cancer Tissues

Molecular Cancer Research : MCR
Stefano CacciatoreMassimo Loda

Abstract

Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively. Classes and physical-chemical characteristics of the metabolites preserved in FFPE material were characterized and related to their preservation or loss following fixation and embedding. Metabolite classes were differentially preserved in archival FFPE tissues, regardless of the age of the block, compared with matched frozen specimen, ranging from maximal preservation of fatty acids (78%) to loss of the majority of peptides and steroids. Generally, FFPE samples showed a decrease of metabolites with functional groups, such as carboxamide. As an adjunct technique, metabolic profiles were also obtained in situ from FFPE tissue sections where met...Continue Reading

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Citations

Dec 7, 2017·Prostate Cancer and Prostatic Diseases·Annika KurreckLeo L Cheng
Dec 13, 2017·Cold Spring Harbor Perspectives in Medicine·Giorgia Zadra, Massimo Loda
Feb 11, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Filipe Fideles Duarte-AndradeFelipe Paiva Fonseca
Feb 26, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Aline Fernanda CruzRicardo Santiago Gomez
Feb 23, 2019·Diagnostics·Marouane KdadraEric Schiffer
Feb 13, 2019·Molecular Cancer Research : MCR·Elizabeth C RandallNathalie Y R Agar
Jan 27, 2021·Journal of Pharmaceutical and Biomedical Analysis·Magdalena Buszewska-ForajtaMichał J Markuszewski

Related Concepts

Malignant Neoplasm of Prostate
Retrospective Studies
Mass Spectrometry
Paraffin Embedding
Tissue Fixation
Cell Line, Tumor
Metabolomics
Biological Markers
Fatty Acids
Gene Expression

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