Metabolic profiling of femoral muscle from rats at different periods of time after death

PloS One
Tieshuai DuYiwen Shen

Abstract

Clarification of postmortem metabolite changes can help characterize the process of biological degradation and facilitate investigations of forensic casework, especially in the estimation of postmortem interval (PMI). Metabolomics can provide information on the molecular profiles of tissues, which can aid in investigating postmortem metabolite changes. In this study, liquid chromatography-mass spectrometric (LC-MS) analysis was performed to identify the metabolic profiles of rat femoral muscle at ten periods of time after death within 168 h. The results obtained by reversed-phase liquid chromatography (RPLC)- and hydrophilic interaction liquid chromatography (HILIC)- electrospray ionization (ESI±) have revealed more than 16,000 features from all four datasets. Furthermore, 915 of these features were identified using an in-house database. Principal component analysis (PCA) demonstrated the time-specific features of molecular profiling at each period of time after death. Moreover, results from partial least squares projection to latent structures-discriminant analysis (PLS-DA) disclosed a strong association of metabolic alterations of at least 59 metabolites with the time since death, especially within 48 h after death, which exp...Continue Reading

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Citations

May 10, 2019·Metabolomics : Official Journal of the Metabolomic Society·Emanuela LocciErnesto d'Aloja
Oct 19, 2019·Analytical Chemistry·Carolina González-RianoCoral Barbas

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Methods Mentioned

BETA
nuclear magnetic resonance
PCA

Software Mentioned

OPLS
XCMS
ProteoWizard MSConvert
SIMCA
PLS
DA

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