Metabolic Profiling of Geobacter sulfurreducens during Industrial Bioprocess Scale-Up

Applied and Environmental Microbiology
Howbeer MuhamadaliRoyston Goodacre

Abstract

During the industrial scale-up of bioprocesses it is important to establish that the biological system has not changed significantly when moving from small laboratory-scale shake flasks or culturing bottles to an industrially relevant production level. Therefore, during upscaling of biomass production for a range of metal transformations, including the production of biogenic magnetite nanoparticles by Geobacter sulfurreducens, from 100-ml bench-scale to 5-liter fermentors, we applied Fourier transform infrared (FTIR) spectroscopy as a metabolic fingerprinting approach followed by the analysis of bacterial cell extracts by gas chromatography-mass spectrometry (GC-MS) for metabolic profiling. FTIR results clearly differentiated between the phenotypic changes associated with different growth phases as well as the two culturing conditions. Furthermore, the clustering patterns displayed by multivariate analysis were in agreement with the turbidimetric measurements, which displayed an extended lag phase for cells grown in a 5-liter bioreactor (24 h) compared to those grown in 100-ml serum bottles (6 h). GC-MS analysis of the cell extracts demonstrated an overall accumulation of fumarate during the lag phase under both culturing condi...Continue Reading

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Citations

Oct 10, 2015·Microbial Cell Factories·Howbeer MuhamadaliRoyston Goodacre
Jul 19, 2020·Applied and Environmental Microbiology·Richard L KimberJonathan R Lloyd

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